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AB250319

Anti-Seladin 1 antibody [EPR10743] - BSA and Azide free

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Rabbit Recombinant Monoclonal Seladin 1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

대체 명칭 보기

KIAA0018, DHCR24, Delta(24)-sterol reductase, 24-dehydrocholesterol reductase, 3-beta-hydroxysterol Delta-24-reductase, Diminuto/dwarf1 homolog, Seladin-1

1 이미지
Western blot - Anti-Seladin 1 antibody [EPR10743] - BSA and Azide free (AB250319)
  • WB

Lab

Western blot - Anti-Seladin 1 antibody [EPR10743] - BSA and Azide free (AB250319)

This data was developed using the same antibody clone in a different buffer formulation (ab181062).

Lanes 1 - 3 : Merged signal (red and green). Green - ab181062 observed at 51 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab181062 was shown to react with DHCR24 in A431 wild-type cells in Western blot. Loss of signal was observed when DHCR24 knockout sample was used. A431 wild-type and DHCR24 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab181062 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Seladin 1 antibody [EPR10743] (<a href='/ko/products/primary-antibodies/seladin-1-antibody-epr10743-ab181062'>ab181062</a>) at 1/2000 dilution

Lane 1:

Wild-type A431 whole cell lysate at 20 µg

Lane 2:

DHCR24 knockout A431 whole cell lysate at 20 µg

Lane 2:

Western blot - Human DHCR24 knockout A-431 cell line (<a href='/ko/products/cell-lines/human-dhcr24-knockout-a-431-cell-line-ab262480'>ab262480</a>)

Lane 3:

HepG2 whole cell lysate at 20 µg

Predicted band size: 60 kDa

Observed band size: 51 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10743

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

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Anti-DHCR7 antibody

Western blot - Anti-DHCR7 antibody (AB103296)

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Primary Antibodies

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Primary Antibodies

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Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (AB317628)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

ab250319 is the carrier-free version of ab181062.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Seladin 1 also known as DHCR24 (3β-Hydroxysteroid-Δ24 reductase) is an enzyme with a molecular mass of approximately 54 kDa. It plays a role in cholesterol biosynthesis by catalyzing the reduction of a double bond at the C-24 position in sterol precursors. Seladin 1 is expressed in various tissues with high expression in the brain liver and adrenal gland. The enzyme contributes to the maintenance of cellular cholesterol homeostasis and provides neuroprotective functions.
Biological function summary

Seladin 1 plays an important role in cellular processes particularly in response to oxidative stress. The protein is not part of a larger complex but functions independently to reduce highly reactive oxygen species levels in cells. It helps in maintaining cellular integrity and contributes to the survival of neuronal cells under stress conditions. Additionally it provides resistance to oxidative stress-induced apoptosis by modulating intracellular antioxidative defense mechanisms.

Pathways

Seladin 1 plays an integral role in the cholesterol biosynthesis pathway and is closely associated with the mevalonate pathway. Its activity directly influences the availability of cholesterol and other sterols impacting membrane fluidity and cellular functions dependent on sterol concentrations. The enzyme interacts with related proteins such as HMG-CoA reductase an important regulatory enzyme within the cholesterol synthesis pathway. These interactions ensure that cellular cholesterol levels are well-regulated to meet physiological demands.

Seladin 1 has been implicated in Alzheimer's disease and certain cancers. In Alzheimer's disease a reduction in Seladin 1 expression correlates with increased susceptibility to amyloid-beta toxicity while its overexpression shows a protective effect. This relationship ties Seladin 1 to amyloid precursor protein processing. In some cancers altered Seladin 1 expression affects tumor growth and progression where it may interact with proteins involved in cell survival pathways. This dual role in both neurodegenerative and cancerous conditions highlights the importance of Seladin 1 in human health.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis (PubMed : 11519011, PubMed : 21671375, PubMed : 22178193, PubMed : 25637936). In addition to its cholesterol-synthesizing activity, can protect cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress (PubMed : 11007892, PubMed : 22010141). Also protects against amyloid-beta peptide-induced apoptosis (PubMed : 11007892).
See full target information DHCR24
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Product promise

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