AB121276

Anti-SELK antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal SELK antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SELENOK aa 1 to C-terminus.

대체 명칭 보기

SELK, HSPC030, HSPC297, SELENOK, Selenoprotein K, SelK

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human testis tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human liver tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Supplier Data

Immunocytochemistry - Anti-SELK antibody (AB121276)

Immunofluorescent staining of U-251 MG (Human brain glioma) cells labeling SELK with ab121276 at 0.25 ug/ml (green).

Supplier Data

Western blot - Anti-SELK antibody (AB121276)

Western blot analysis of HepG2 cells labeling SELK with ab121276 at 0.04 ug/ml.

All lanes:

Western blot - Anti-SELK antibody (ab121276) at 0.04 µg/mL

All lanes:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 11 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SELENOK aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6D0

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein SELK also known as selenoprotein K plays an important role in cellular processes. SELK is a small protein with a mass of about 11.4 kDa. It is characterized by the presence of the amino acid selenocysteine which is rare and important for its function. SELK produces in various tissues including heart skeletal muscle and liver. It locates in the endoplasmic reticulum membrane where it interacts with other proteins to contribute to molecular processes essential for cell homeostasis.

Biological function summary

SELK influences the regulation of calcium flux in cells affecting cellular signaling and immune responses. It is not a stand-alone actor but associates with other proteins to form multi-protein complexes that facilitate these cellular functions. SELK participates in processes like antioxidant defense and endoplasmic reticulum stress response which protects cells from damage. By modulating the immune response SELK plays an important role in maintaining balance in the immune system.

Pathways

This protein interacts in the oxidative stress pathways and plays a role in calcium signaling. In oxidative stress pathways SELK helps protect cells against reactive oxygen species maintaining cellular health. It works closely with proteins such as selenoprotein S and other antioxidant defense proteins. In calcium signaling SELK helps regulate calcium ion flow across cellular membranes supporting various cellular functions essential for those pathways.

SELK connects to cardiovascular diseases and autoimmune disorders. Its involvement in regulating immune responses and calcium flux associates with the pathogenesis of these conditions. In cardiovascular diseases abnormal SELK expression may contribute to heart abnormalities by interacting with calcium-modulating proteins like calnexin. In autoimmune disorders a dysregulated SELK function may affect immune balance and lead to immune system malfunction. Understanding SELK interactions gives insights into these diseases and potential therapeutic approaches.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for Ca(2+) flux in immune cells and plays a role in T-cell proliferation and in T-cell and neutrophil migration (By similarity). Involved in endoplasmic reticulum-associated degradation (ERAD) of soluble glycosylated proteins (PubMed : 22016385). Required for palmitoylation and cell surface expression of CD36 and involved in macrophage uptake of low-density lipoprotein and in foam cell formation (By similarity). Together with ZDHHC6, required for palmitoylation of ITPR1 in immune cells, leading to regulate ITPR1 stability and function (PubMed : 25368151). Plays a role in protection of cells from ER stress-induced apoptosis (PubMed : 20692228). Protects cells from oxidative stress when overexpressed in cardiomyocytes (PubMed : 16962588).

See full target information SELENOK

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:8030 PubMed40877267

2025

Organic-inorganic covalent selenium reversing ischemic reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zushuang Xiong,Guanning Huang,Jia-Run Huang,Ying Liu,Lizhen He,Tianfeng Chen

Cell and tissue research 401:59-68 PubMed40323407

2025

Histopathological analysis of respiratory muscles in patients with acute COVID-19 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Steingruber,Simona Handtke,Franziska Schweiger,Stefan Schiele,Bruno Märkl,Marco Koch

International journal of nanomedicine 19:2071-2090 PubMed38476275

2024

MSC-Derived Exosomes Mitigate Myocardial Ischemia/Reperfusion Injury by Reducing Neutrophil Infiltration and the Formation of Neutrophil Extracellular Traps.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Feng,Xue Bao,Jinxuan Zhao,Lina Kang,Xuan Sun,Biao Xu

Redox biology 57:102500 PubMed36252341

2022

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyue You,Fan Wu,Meilin Gao,Mengyue Chen,Shu Zeng,Yang Zhang,Wei Zhao,Danyang Li,Li Wei,Xiong Z Ruan,Yaxi Chen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SELK antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

Immunocytochemistry - Anti-SELK antibody (AB121276)

Western blot - Anti-SELK antibody (AB121276)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise