AB223721

Anti-SELS antibody

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Polyclonal SELS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SELENOS aa 50 to C-terminus.

대체 명칭 보기

SELS, VIMP, AD-015, SBBI8, SELENOS, Selenoprotein S, SelS, VCP-interacting membrane protein

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELS antibody (AB223721)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELS antibody (AB223721)

Paraffin embedded human pancreas tissue stained for SELS with ab223721 (1/200 dilution) in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SELS antibody (AB223721)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SELS antibody (AB223721)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SELS (green) using ab223721 (4 μg/ml) in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELS antibody (AB223721)

Paraffin embedded human small intestine tissue stained for SELS with ab223721 (1/200 dilution) in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SELS antibody (AB223721)

All lanes:

Western blot - Anti-SELS antibody (ab223721) at 1/100 dilution

All lanes:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 21 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SELENOS aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BQE4

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Selenoprotein S (SELS) also known as VIMP has a significant role in promoting the degradation of misfolded proteins in the endoplasmic reticulum (ER). It is part of the selenoprotein family incorporating selenium in the form of selenocysteine. The protein has a molecular mass of approximately 22 kDa. SELS localizes to the membrane of the ER and is expressed broadly across various tissues showing higher levels in liver and muscle.

Biological function summary

SELS participates actively in the regulation of ER stress response and inflammation. It functions as an important component of the ER-associated degradation (ERAD) pathway mediating the extraction and degradation of faulty proteins. This activity stems from its ability to interact with ubiquitin ligase complexes aiding in protein quality control. Its role is integral for maintaining cellular homeostasis and preventing accumulation of protein aggregates.

Pathways

SELS engages profoundly with the oxidative stress and inflammatory pathways. It modulates the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signaling pathway which influences inflammation and immune responses. SELS also interfaces with other proteins like p97/VCP and Derlin-1 which assist in the ERAD process demonstrating its connectivity within cellular stress response pathways.

SELS has connections to metabolic syndrome and inflammatory diseases. Altered expression levels of SELS are linked to the development of type 2 diabetes and obesity by influencing inflammatory mechanisms. SELS interacts with inflammatory cytokines like tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) highlighting its relevance in modulating immune responses linked to these conditions.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Involved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination.

See full target information SELENOS

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JCI insight 9: PubMed39315548

2024

Pyruvate metabolism dictates fibroblast sensitivity to GLS1 inhibition during fibrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Greg Contento,Jo-Anne Am Wilson,Brintha Selvarajah,Manuela Platé,Delphine Guillotin,Valle Morales,Marcello Trevisani,Vanessa Pitozzi,Katiuscia Bianchi,Rachel C Chambers

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SELS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELS antibody (AB223721)

Immunocytochemistry/ Immunofluorescence - Anti-SELS antibody (AB223721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELS antibody (AB223721)

Western blot - Anti-SELS antibody (AB223721)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise