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AB285132

Anti-Semaphorin 4D/CD100 antibody [EPR23866-52] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Semaphorin 4D/CD100 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-Semaphorin 4D/CD100 antibody [EPR23866-52] - BSA and Azide free (Detector) (AB285132)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Semaphorin 4D/CD100 antibody [EPR23866-52] - BSA and Azide free (Detector) (AB285132)

Example of Human Semaphorid 4D/CD10 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23866-52

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB282868

Human Semaphorid 4D/CD10 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Semaphorid 4D/CD10 ELISA Kit (AB282868)

  • 0
  • 0
Sample Prep & Detection Kits

AB201800

DyLight® 550 Conjugation Kit (Fast) - Lightning-Link®

Conjugation - DyLight® 550 Conjugation Kit (Fast) - Lightning-Link® (AB201800)

  • 5
  • 2
Sample Prep & Detection Kits

AB218260

Oligonucleotide Conjugation Kit

Conjugation - Oligonucleotide Conjugation Kit (AB218260)

  • 0
  • 0
Primary Antibodies

AB278348

Anti-HRG antibody [EPR24184-134] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-HRG antibody [EPR24184-134] - BSA and Azide free (Capture) (AB278348)

  • 0
  • 0
Primary Antibodies

AB305123

Anti-S100A11 antibody [EPR25825-112] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-S100A11 antibody [EPR25825-112] - BSA and Azide free (Detector) (AB305123)

  • 0
  • 0
Primary Antibodies

AB245045

Anti-PLA2G7 antibody [EPR20360-245] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-PLA2G7 antibody [EPR20360-245] - BSA and Azide free (Detector) (AB245045)

  • 0
  • 0
Primary Antibodies

AB285097

Anti-Cathepsin Z antibody [EPR24460-104] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Cathepsin Z antibody [EPR24460-104] - BSA and Azide free (Detector) (AB285097)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab285132 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Semaphorin 4D/CD100. The recommended pair for sandwich ELISA is:
Capture: ab285026, Human Semaphorin 4D/CD100 Capture Antibody (unconjugated)
Detector: ab285132, Human Semaphorin 4D/CD100 Detector Antibody (unconjugated)
The reference range value is 78 - 5000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Semaphorin 4D also known as CD100 is a transmembrane protein that plays important roles in cellular signaling. It belongs to the semaphorin protein family known for their role in axonal guidance. CD100 has a molecular mass of approximately 150 kDa. This protein expresses mainly in the immune system including T cells and B cells but can also be found in certain neurons and endothelial cells. It can exist in both a membrane-bound form and a soluble form which arise from proteolytic cleavage.
Biological function summary

CD100 influences various cellular functions by interacting with multiple receptors such as Plexin-B1 and CD72 as part of a larger signaling complex. It contributes to immune response regulation by promoting T-cell activation and B-cell aggregation which are essential for adaptive immunity. In the nervous system it guides neural development including axon growth and synaptic plasticity. CD100's interaction with Plexin-B1 plays a significant role in these developmental processes often affecting cell morphology and movement.

Pathways

Semaphorin 4D is involved in both immune and neural signaling pathways. In the immune system it influences the PI3K-Akt signaling pathway which impacts cell survival and immune cell activation. It associates with pathways involving Plexin-B1 and RhoA to regulate cytoskeletal dynamics affecting cell motility and growth. Additionally CD100's role in neural pathways intersects with proteins like neuropilin-1 influencing semaphorin-mediated signaling essential for neural connectivity.

Semaphorin 4D has links to cancer and autoimmune diseases. High expression levels of CD100 are observed in several tumor types where it might promote tumor growth and metastasis through its signaling pathways. In autoimmune conditions like multiple sclerosis CD100 affects immune cell interactions and can exacerbate inflammatory responses. Its involvement with proteins such as CD72 and Plexin-B1 in these conditions highlights its contribution to pathogenesis and potential as a therapeutic target.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed : 20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed : 19788569). Promotes the migration of cerebellar granule cells (PubMed : 16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed : 8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed : 16055703).
See full target information SEMA4D

대체 명칭 보기

CD100, C9orf164, SEMAJ, SEMA4D, Semaphorin-4D, A8, BB18, GR3
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Product promise

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