AB186021

Anti-SEPT7 antibody

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(1 리뷰)

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(4 제품이 사용된 논문 )

Rabbit Polyclonal SEPT7 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SEPTIN7 aa 350-450.

대체 명칭 보기

CDC10, SEPT7, SEPTIN7, Septin-7, CDC10 protein homolog

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB186021)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB186021)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human osteosarcoma labeling SEPT7 with ab186021 at a 1/1000 dilution.

Immunoprecipitation - Anti-SEPT7 antibody (AB186021)

IP

Supplier Data

Immunoprecipitation - Anti-SEPT7 antibody (AB186021)

ab186021 at 1 μg/ml detecting SEPT7 in HeLa whole cell lysate by WB following IP. ab186021 was used for IP at 6 μg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection : Chemiluminescence with an exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-SEPT7 antibody (ab186021)

Predicted band size: 51 kDa

true

Exposure time: 1s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB186021)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB186021)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse teratoma labeling SEPT7 with ab186021 at a 1/1000 dilution.

Western blot - Anti-SEPT7 antibody (AB186021)

WB

Supplier Data

Western blot - Anti-SEPT7 antibody (AB186021)

All lanes:

Western blot - Anti-SEPT7 antibody (ab186021) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 51 kDa

true

Exposure time: 1s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

Immunogen

Synthetic Peptide within Human SEPTIN7 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB244434

Anti-SEPT7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

0
0

Primary Antibodies

AB186010

Anti-SEPT7 antibody

Immunoprecipitation - Anti-SEPT7 antibody (AB186010)

5
2

Primary Antibodies

AB175229

Anti-SEPT7 antibody [EPR12610]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] (AB175229)

0
0

Primary Antibodies

AB245761

Anti-SEPT7 antibody [EPR12610] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] - BSA and Azide free (AB245761)

0
0

보완 제품

Primary Antibodies

AB179436

Anti-Septin 2 antibody [EPR12123]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12123] (AB179436)

0
0

Primary Antibodies

AB191404

Anti-Septin 8 antibody [EPR16099]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Septin 8 antibody [EPR16099] (AB191404)

5
1

Primary Antibodies

AB108629

Anti-TUBA1B antibody [EPR1333] - Loading Control

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

0
0

Primary Antibodies

AB40815

Anti-EGFR (phospho Y1068) antibody [EP774Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)

4
14

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SEPT7 also known as Septin-7 or CDC10 is a protein that belongs to the septin family. Septins are GTP-binding proteins known for forming hetero-oligomeric complexes. SEPT7 has an approximate molecular mass of 52 kDa. It is expressed in a variety of tissues including the brain heart liver and kidneys among others. Mechanically SEPT7 plays a role in forming filaments and rings serving as scaffolds for other proteins and contributing to the structural organization of the cytoskeleton.

Biological function summary

In cellular environments SEPT7 is a part of the septin complex which is important for maintaining cell shape and polarity. It participates in processes involving cell division particularly during cytokinesis where it helps in forming the contractile ring necessary for dividing cells. SEPT7 also interacts with actin and microtubules facilitating cell movement and intracellular transport. This protein's dynamics contribute to the stability and segregation of cellular components during cell division.

Pathways

SEPT7 plays a role in the regulation of the actin cytoskeleton and cell cycle pathways. It interacts with proteins like actin an important component in cellular motion and structure. SEPT7's involvement in the cellular cycle pathway connects it to proteins such as CDC42 which regulate cell proliferation and differentiation. These interactions make SEPT7 significant in controlling the proper progression through different cell cycle phases.

SEPT7 has connections to cancer and neurological disorders. Its disrupted expression has been linked to tumorigenesis as abnormalities in cell division and cytoskeletal organization can promote malignant transformation. In neurological contexts SEPT7 interacts with presynaptic proteins and its dysregulation is related to neurodegenerative diseases such as Alzheimer's disease. Deficiencies in SEPT7 may lead to improper synaptic functioning contributing to disease pathology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed : 25588830).

See full target information SEPTIN7

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of advanced research 73:517-533 PubMed39111626

2024

Pharmacological inhibition of Septins with Forchlorfenuron attenuates thrombus formation in experimental thrombotic mice models with modulating multiple signaling pathways in platelets.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Hao,Minghui Yan,Reyisha Tuerhong,Luying Zhang,Zhen Zhang,Habib Alam,Jun Wu,Yuanhua Qin,Feng Zhao,Lei Shi

Cancers 13: PubMed34572914

2021

HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Barreiro-Alonso,Mónica Lamas-Maceiras,Lidia Lorenzo-Catoira,Mercedes Pardo,Lu Yu,Jyoti S Choudhary,M Esperanza Cerdán

Molecular medicine reports 21:2427-2434 PubMed32323792

2020

Relationship between autophagy, apoptosis and endoplasmic reticulum stress induced by melatonin in osteoblasts by septin7 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cui Cui,Tao Lin,Zunlei Gong,Yue Zhu

Molecular medicine reports 17:4817-4822 PubMed29344665

2018

Overexpression of septin-7 inhibits melatonin-induced cell apoptosis in human fetal osteoblastic cells via suppression of endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotong Meng,Yue Zhu,Lin Tao,Sichao Zhao,Shui Qiu

제품이 사용된 논문 모두 보기

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Product promise

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