AB244434

Anti-SEPT7 antibody

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Rabbit Polyclonal SEPT7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEPTIN7 aa 150-300.

대체 명칭 보기

CDC10, SEPT7, SEPTIN7, Septin-7, CDC10 protein homolog

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

Paraffin-embedded human skeletal muscle tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis. No positivity in myocytes, as expected.

Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody (AB244434)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody (AB244434)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SEPT7 (green) using ab244434 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

Paraffin-embedded human fallopian tube tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

Paraffin-embedded human cerebral cortex tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

Paraffin-embedded human testis tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

Paraffin-embedded human lymph node tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-SEPT7 antibody (AB244434)

All lanes:

Western blot - Anti-SEPT7 antibody (ab244434) at 0.4 µg/mL

All lanes:

U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate

Predicted band size: 51 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SEPTIN7 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q16181

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SEPT7 also known as Septin-7 or CDC10 is a protein that belongs to the septin family. Septins are GTP-binding proteins known for forming hetero-oligomeric complexes. SEPT7 has an approximate molecular mass of 52 kDa. It is expressed in a variety of tissues including the brain heart liver and kidneys among others. Mechanically SEPT7 plays a role in forming filaments and rings serving as scaffolds for other proteins and contributing to the structural organization of the cytoskeleton.

Biological function summary

In cellular environments SEPT7 is a part of the septin complex which is important for maintaining cell shape and polarity. It participates in processes involving cell division particularly during cytokinesis where it helps in forming the contractile ring necessary for dividing cells. SEPT7 also interacts with actin and microtubules facilitating cell movement and intracellular transport. This protein's dynamics contribute to the stability and segregation of cellular components during cell division.

Pathways

SEPT7 plays a role in the regulation of the actin cytoskeleton and cell cycle pathways. It interacts with proteins like actin an important component in cellular motion and structure. SEPT7's involvement in the cellular cycle pathway connects it to proteins such as CDC42 which regulate cell proliferation and differentiation. These interactions make SEPT7 significant in controlling the proper progression through different cell cycle phases.

SEPT7 has connections to cancer and neurological disorders. Its disrupted expression has been linked to tumorigenesis as abnormalities in cell division and cytoskeletal organization can promote malignant transformation. In neurological contexts SEPT7 interacts with presynaptic proteins and its dysregulation is related to neurodegenerative diseases such as Alzheimer's disease. Deficiencies in SEPT7 may lead to improper synaptic functioning contributing to disease pathology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed : 25588830).

See full target information SEPTIN7

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

American journal of physiology. Cell physiology 319:C93-C104 PubMed31747529

2019

Downregulation of AAA-domain-containing protein 2 restrains cancer stem cell properties in esophageal squamous cell carcinoma via blockade of the Hedgehog signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nuo Li,Yang Yu,Baoming Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEPT7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody (AB244434)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (AB244434)

Western blot - Anti-SEPT7 antibody (AB244434)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise