Anti-Septin 2 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody (AB88657)

ab88657 at 10μg/ml staining SEPT2 in HeLa cells by Immunofluorescence.

• WB

Unknown

Western blot - Anti-Septin 2 antibody (AB88657)

All lanes:

Western blot - Anti-Septin 2 antibody (ab88657) at 1 µg/mL

All lanes:

Human spleen tissue lysate at 50 µg

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

• ICC/IF

CiteAb

Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody (AB88657)

Immunocytochemistry-immunofluorescence using Anti-Septin 2 antibody, ab88657. Publication image from Peng, J. et al., 2016, Nat Commun, 27417143. Legend direct from paper.

Septin 9 increases in JFH-1-infected cells and regulates septin 2 and microtubules filaments.(a) Immunoblot of septin 9 and core in Huh7.5 cells infected or not with JFH-1 for 24, 48 and 72 h. Actin was used as a loading control. The bar graph presents the densitometry analysis of the immunoblots from three independent experiments. (b) Huh7.5 cells transfected with non-targeting (control) or septin 9 siRNA (si3) for 24 h then infected or not with HCV JFH-1 for 72 h then stained for septin 9 (green) and core (red). (c) Immunoblot of septin 9 and core in Huh7.5 cells transfected with non-targeting (control) or septin 9 siRNA (si1 or si3) then infected with HCV JFH-1 for 72 h. (d) Huh7.5 treated as in b and stained for septin 9 (green) and septin 2 (red). (e) Dot rectangles in d are presented in higher magnification. (f) Bar graph shows Pearson's correlation coefficient (Rr) of septin 9 and septin 2 calculated in 30 cells from 2 independent experiments. (g) Immunoblot of septin 2, septin 9 and core in Huh7.5 cells treated as described in b. Actin was used as a loading control. Bar graph presents septin 2 expression from three independent experiments. (h) Huh7.5 cells were treated as described in b and stained for microtubules (MTs) with β tubulin (red) and septin 9 (green). (i) Dot squares in h present in higher magnification. (j) Bar graph shows Pearson's correlation coefficient (Rr) analysis of septin 9 and MTs calculated in 30 cells from two independent experiments. Values are means±s.e.m. Student's t-test was used. *P<0.05, ***P<0.0001. Scale bar, 10 µm.

• ICC/IF

CiteAb

Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody (AB88657)

Immunocytochemistry-immunofluorescence using Anti-Septin 2 antibody, ab88657. Publication image from Peng, J. et al., 2016, Nat Commun, 27417143. Legend direct from paper.

Septin 9 increases in JFH-1-infected cells and regulates septin 2 and microtubules filaments.(a) Immunoblot of septin 9 and core in Huh7.5 cells infected or not with JFH-1 for 24, 48 and 72 h. Actin was used as a loading control. The bar graph presents the densitometry analysis of the immunoblots from three independent experiments. (b) Huh7.5 cells transfected with non-targeting (control) or septin 9 siRNA (si3) for 24 h then infected or not with HCV JFH-1 for 72 h then stained for septin 9 (green) and core (red). (c) Immunoblot of septin 9 and core in Huh7.5 cells transfected with non-targeting (control) or septin 9 siRNA (si1 or si3) then infected with HCV JFH-1 for 72 h. (d) Huh7.5 treated as in b and stained for septin 9 (green) and septin 2 (red). (e) Dot rectangles in d are presented in higher magnification. (f) Bar graph shows Pearson's correlation coefficient (Rr) of septin 9 and septin 2 calculated in 30 cells from 2 independent experiments. (g) Immunoblot of septin 2, septin 9 and core in Huh7.5 cells treated as described in b. Actin was used as a loading control. Bar graph presents septin 2 expression from three independent experiments. (h) Huh7.5 cells were treated as described in b and stained for microtubules (MTs) with β tubulin (red) and septin 9 (green). (i) Dot squares in h present in higher magnification. (j) Bar graph shows Pearson's correlation coefficient (Rr) analysis of septin 9 and MTs calculated in 30 cells from two independent experiments. Values are means±s.e.m. Student's t-test was used. *P<0.05, ***P<0.0001. Scale bar, 10 µm.

• WB

CiteAb

Western blot - Anti-Septin 2 antibody (AB88657)

Western Blotting using Anti-Septin 2 antibody, ab88657. Publication image from Peng, J. et al., 2016, Nat Commun, 27417143. Legend direct from paper.

Septin 9 increases in JFH-1-infected cells and regulates septin 2 and microtubules filaments.(a) Immunoblot of septin 9 and core in Huh7.5 cells infected or not with JFH-1 for 24, 48 and 72 h. Actin was used as a loading control. The bar graph presents the densitometry analysis of the immunoblots from three independent experiments. (b) Huh7.5 cells transfected with non-targeting (control) or septin 9 siRNA (si3) for 24 h then infected or not with HCV JFH-1 for 72 h then stained for septin 9 (green) and core (red). (c) Immunoblot of septin 9 and core in Huh7.5 cells transfected with non-targeting (control) or septin 9 siRNA (si1 or si3) then infected with HCV JFH-1 for 72 h. (d) Huh7.5 treated as in b and stained for septin 9 (green) and septin 2 (red). (e) Dot rectangles in d are presented in higher magnification. (f) Bar graph shows Pearson's correlation coefficient (Rr) of septin 9 and septin 2 calculated in 30 cells from 2 independent experiments. (g) Immunoblot of septin 2, septin 9 and core in Huh7.5 cells treated as described in b. Actin was used as a loading control. Bar graph presents septin 2 expression from three independent experiments. (h) Huh7.5 cells were treated as described in b and stained for microtubules (MTs) with β tubulin (red) and septin 9 (green). (i) Dot squares in h present in higher magnification. (j) Bar graph shows Pearson's correlation coefficient (Rr) analysis of septin 9 and MTs calculated in 30 cells from two independent experiments. Values are means±s.e.m. Student's t-test was used. *P<0.05, ***P<0.0001. Scale bar, 10 µm.

false

• WB

CiteAb

Western blot - Anti-Septin 2 antibody (AB88657)

Western Blotting using Anti-Septin 2 antibody, ab88657. Publication image from Peng, J. et al., 2016, Nat Commun, 27417143. Legend direct from paper.

Sodium oleate treatment increases septin 9 and LDs in the perinuclear region and in LD-rich fractions.(a) Huh7 cells were grown for 24 h. The culture medium was supplemented with 0, 50, 100 or 200 µM sodium oleate complex for 24 h then stained for septin 9 (green) and LDs (red). The white squares indicate the zoomed area shown as a 3D reconstruction image with a white background. (b) Immunoblot analysis of septin 9 and PLIN2 in cells treated as in a. Bar graphs below show the analysis from three independent experiments. (c) Huh7 cells were treated or not with sodium oleate at 100 µM were submitted to membrane flotation assay and analysed by western blot for septin 9, septin 2, PLIN2 and Calnexin. (d) Densitometry analysis of protein expression profile from western blot in c is shown. Values are means±s.e.m. Student's t-test was used. *P<0.05, **P<0.001. Scale bar, 10 µm.

false