AB307432

Anti-Serine Palmitoyltransferase antibody [EPR26954-39]

KD Validated
20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Serine Palmitoyltransferase antibody. Suitable for ICC/IF, WB and reacts with Rat, Human, Mouse samples. Cited in 2 publications.

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (AB307432)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (AB307432)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized MCF7 (human breast adenocarcinoma epithelial cellЈ© cells lABelling Serine Palmitoyltransferase with ab307432 at 1/50 (10.64 ug/ml) dilution, followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in MCF7 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/ml dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (AB307432)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized PC-12 (rat adrenal gland pheochromocytoma small irregularly shaped cells) cells lABelling Serine Palmitoyltransferase with ab307432 at 1/50 (10.64 ug/ml) dilution, followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Supplier Data

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (AB307432)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTLow expression : mouse skeletal muscle (PMID : 17023427) The expression pattern and molecular weight is consistent to what has been described in the literature (PMID : 17023427) Exposure time : 26 seconds

All lanes:

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (ab307432) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate 20 μg

Lane 2:

Human liver tissue lysate 20 μg

Lane 3:

Mouse placenta tissue lysate 20 μg

Lane 4:

Mouse liver tissue lysate 20 μg

Lane 5:

Mouse skeletal muscle tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (AB307432)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTThe molecular weight is consistent to what has been described in the literature (PMID : 17023427) Exposure time : 59 seconds

All lanes:

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (ab307432) at 1/1000 dilution

Lane 1:

Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 μg

Lane 2:

Hela transfected with siRNA specifically targeti Serine Palmitoyltransferase whole cell lysate 20 μg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 4:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg

Lane 5:

RAW 264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 μg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 59s

Supplier Data

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (AB307432)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTThis antibody does not cross-react with human Serine Palmitoyltransferase 3. Exposure time : 5.5 seconds

All lanes:

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (ab307432) at 1/1000 dilution

Lane 1:

His-tagged human Serine palmitoyltransferase 2 recombinant protein 10 ng

Lane 2:

His-tagged human Serine palmitoyltransferase 3 recombinant protein 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 5.5s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26954-39

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Serine palmitoyltransferase (SPT) sometimes referred to as SPTLC is an enzyme that plays an important role in the initial step of sphingolipid biosynthesis. It catalyzes the condensation of serine and palmitoyl-CoA yielding 3-ketodihydrosphingosine. This enzyme complex consists of subunits SPTLC1 SPTLC2 and sometimes SPTLC3 with a molecular weight around 53 kDa for SPTLC1. Expression of SPT is seen in various tissues including the liver and nervous system supporting its critical functions across the body.

Biological function summary

Serine palmitoyltransferase is involved in sphingolipid metabolism contributing to the formation of essential cellular components. It acts as a part of a larger enzyme complex responsible for generating sphingolipid precursors. These sphingolipids play a role in membrane structure and cell signaling affecting processes like cell growth and apoptosis. The enzyme therefore influences fundamental cellular phenomena demonstrating its widespread relevance in biochemistry and cell biology.

Pathways

Serine palmitoyltransferase is vital in the de novo sphingolipid biosynthesis pathway where it initiates the creation of long-chain bases the core of most sphingolipids. It also links to the lipid metabolism pathway sharing interactions with proteins like ceramide synthase. By producing the starting materials for sphingolipids SPT integrates deeply into lipid regulation and signaling pathways affecting how cells communicate and respond to their environment.

Serine palmitoyltransferase has connections to disorders like hereditary sensory and autonomic neuropathy type I (HSAN1) and metabolic syndromes. Mutations in the SPTLC1 subunit can lead to HSAN1 illustrating its direct involvement in nerve function and health. Additionally altered sphingolipid metabolism implicates SPT in metabolic syndromes where it interacts with proteins like ceramide influencing insulin resistance and lipid homeostasis. These connections emphasize the enzyme's significance in disease development and potential therapeutic targeting.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases (PubMed : 19416851, PubMed : 19648650, PubMed : 20504773, PubMed : 20920666). The SPT complex is composed of SPTLC1, SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer consisting of SPTLC1 and SPTLC2/SPTLC3 forms the catalytic core (PubMed : 19416851). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference (PubMed : 19416851). The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA (PubMed : 19416851, PubMed : 19648650). The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (PubMed : 19416851, PubMed : 19648650). Crucial for adipogenesis (By similarity).

See full target information SPTLC2

대체 명칭 보기

KIAA0526, LCB2, SPTLC2, Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, Long chain base biosynthesis protein 2a, Serine-palmitoyl-CoA transferase 2, LCB 2, LCB2a, SPT 2

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록and refine your search

Molecular medicine reports 31: PubMed40084708

2025

Effects of natural killer cell‑conditioned medium on UVB‑induced photoaging in human keratinocytes and a human reconstructed skin model.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Ok Lee,Jung Min Lee,Yujin Kim,A Yeon Park,Daewon Yoon,Su Young Kim,Jihye Heo,Seungryel Han,Hyungjin Nam,Hye Jin Shin,Kyeongsoo Jeong,Minju Im,Beom Joon Kim

Cell communication and signaling : CCS 23:124 PubMed40055817

2025

Extracellular vesicular delivery of ceramides from pulmonary macrophages to endothelial cells facilitates chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qiqing Huang,Tutu Kang,Shaoran Shen,Lele Liu,Lili Zhang,Xiaoli Zou,Jianqing Wu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Serine Palmitoyltransferase antibody [EPR26954-39]

Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (AB307432)

Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (AB307432)

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (AB307432)

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (AB307432)

Western blot - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] (AB307432)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise