AB254839

Anti-SERP1 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal SERP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERP1 aa 1-50.

대체 명칭 보기

RAMP4, SERP1, Stress-associated endoplasmic reticulum protein 1, Ribosome-attached membrane protein 4

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERP1 antibody (AB254839)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERP1 antibody (AB254839)

Paraffin-embedded human pancreas tissue stained for SERP1 using ab254839 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SERP1 antibody (AB254839)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SERP1 antibody (AB254839)

PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling SERP1 using ab254839 at 4µg/ml (green) in ICC/IF.

Supplier Data

Western blot - Anti-SERP1 antibody (AB254839)

All lanes:

Western blot - Anti-SERP1 antibody (ab254839) at 0.4 µg/mL

Lane 1:

NIH/3T3 cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 7 kDa

false

Supplier Data

Western blot - Anti-SERP1 antibody (AB254839)

Lane 1:

Western blot - Anti-SERP1 antibody (ab254839)

Lanes 2 - 5:

Western blot - Anti-SERP1 antibody (ab254839) at 0.4 µg/mL

Lane 1:

MW marker

Lane 2:

RT4 cell lysate

Lane 3:

U-251 MG cell lysate

Lane 4:

Human plasma

Lane 5:

Human liver tissue lysate

Predicted band size: 7 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SERP1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6X1

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Stress-Associated Endoplasmic Reticulum Protein 1 (SERP1) also known as Ribosome-Associated Membrane Protein 4 (RAMP4) performs important mechanical functions within the cell. With a mass of about 24 kDa SERP1 assists in the stabilization of newly synthesized polypeptides ensuring their proper folding and maturation within the endoplasmic reticulum. This protein is ubiquitously expressed in many tissues reflecting its broad role in maintaining cellular homeostasis.

Biological function summary

The function of SERP1 involves the interaction with ribosomes and the endoplasmic reticulum's membrane-associated components. SERP1 helps manage and support the correct folding of nascent proteins especially during cellular stress conditions. It does not form part of a larger protein complex but works closely with other stress response proteins to regulate protein synthesis integrity under adverse conditions.

Pathways

Stress response mechanisms and protein synthesis serve as essential biological contexts for SERP1's function. SERP1 is active in the endoplasmic reticulum stress response pathway helping mitigate misfolded protein accumulation during conditions such as unfolded protein response (UPR). It interacts with proteins like BiP/GRP78 which plays a part in these pathways coordinating a cell's adaptation to stress.

The malfunction of SERP1 impacts neurodegenerative diseases and metabolic disorders. Research links SERP1 to conditions such as Alzheimer's disease where inefficiencies in protein handling may worsen pathology. Moreover disruptions in SERP1 activity can associate with metabolic disorders like diabetes involving proteins such as PERK which participates in managing cellular stress responses similar to SERP1.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins.

See full target information SERP1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in cell and developmental biology 13:1521437 PubMed40206403

2025

Identification of disease-stage therapeutic responses of mesenchymal stromal cells retrieved from murine osteoarthritic joints.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Ivanovska,Patrizio Mancuso,Amy Burke,Conor Hennessy,Swarna Raman,Claire Dooley,Steven McLoughlin,Georgina Shaw,Eka Mukeria,Jamie Reilly,Aisling O'Brien,Thomas Ritter,Aideen E Ryan,Raj Kamath,Marc C Levesque,Deborah Van Riet,Karen English,Ian Hawthorne,Brian Johnstone,Derek W Morris,Frank Barry,J Mary Murphy

Molecular medicine reports 25: PubMed35419615

2022

SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β‑catenin/TCF/LEF signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Cai,Zhenhua Sun,Lili Zhang,Hongrui Xu

Molecular therapy oncolytics 19:163-177 PubMed33209976

2020

hsa_circ_0085539 Promotes Osteosarcoma Progression by Regulating miR-526b-5p and SERP1.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Dunwei Wang,Xu Wang,Pengcheng Liu,Ming Yan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SERP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERP1 antibody (AB254839)

Immunocytochemistry/ Immunofluorescence - Anti-SERP1 antibody (AB254839)

Western blot - Anti-SERP1 antibody (AB254839)

Western blot - Anti-SERP1 antibody (AB254839)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise