AB195969

Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker

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Goat Polyclonal SERPINB1/PI2 antibody. Membrane Vesicle marker. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SERPINB1 aa 100-150.

대체 명칭 보기

ELANH2, MNEI, PI2, SERPINB1, Leukocyte elastase inhibitor, LEI, Monocyte/neutrophil elastase inhibitor, Peptidase inhibitor 2, Serpin B1, EI, M/NEI, PI-2

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (AB195969)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (AB195969)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling SERPINB1/PI2 with ab195969 at 5 μg/ml.

Lab

Western blot - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (AB195969)

Western blot : Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker ab195969 staining at 0.5 µg/mL, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 43 kDa in Wild-type A549 cell lysates with no signal observed at this size in SERPINB1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat 800CW and Donkey anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969) at 0.5 µg/mL

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

SERPINB1 knockout A549 at 20 µg

Lane 3:

SK-OV-3 at 20 µg

Lane 4:

HT1080 at 20 µg

Secondary

All lanes:

Donkey anti-Goat 800CW & Donkey anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa,52 kDa

false

Supplier Data

Western blot - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (AB195969)

The fainter 45 kDa band likely represents the acetylated form. Both bands are blocked by the immunizing peptide.

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969) at 0.5 µg/mL

Lane 1:

Human placenta lysate (in RIPA buffer) at 35 µg

Lane 2:

Human placenta lysate (in RIPA buffer) at 35 µg with immunizing peptide

Predicted band size: 43 kDa

Observed band size: 42-45 kDa

true

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SERPINB1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P30740

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SERPINB1 also known as PI2 functions as a serine protease inhibitor. It has an approximate mass of 42 kDa. Serine protease inhibitors play a role in regulating a variety of proteases to maintain cellular homeostasis. This protein mainly expresses in neutrophils epithelial cells and certain immune cells. By inhibiting neutrophil elastase and cathepsin G SERPINB1 protects tissues from protease-driven damage during inflammatory responses.

Biological function summary

SERPINB1 acts to control proteolytic activity and ensures regulated inflammatory responses. It does not form any known complexes but interacts directly with other proteases to modulate their activity. By neutralizing serine proteases SERPINB1 helps in maintaining the balance in immune cell functions. This regulation contributes to the prevention of excessive tissue damage during inflammation emphasizing its role in immune homeostasis.

Pathways

SERPINB1 participates in key regulatory processes such as immune response and protease inhibition pathways. It has a significant influence on pathways involving serine proteases like neutrophil elastase pathways. By interacting with these proteases SERPINB1 modulates their activities ensuring controlled inflammatory reactions and protection against tissue damage.

SERPINB1 links to conditions like chronic obstructive pulmonary disease (COPD) and acute lung injury. These conditions often involve excessive proteolytic activity and SERPINB1 acts to counterbalance this to protect the lung tissue. Additionally the protein shows interactions with other proteins like alpha-1-antitrypsin which also acts as a serine protease inhibitor. The relationship between these proteins highlights their collective role in managing protease activity associated with lung diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis (PubMed : 30692621). Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3 (PubMed : 11747453, PubMed : 30692621). Also acts as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity (PubMed : 23269243). During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation (PubMed : 30692621). When secreted, promotes the proliferation of beta-cells via its protease inhibitory function (PubMed : 26701651).

See full target information SERPINB1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (AB195969)

Western blot - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (AB195969)

Western blot - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (AB195969)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise