Anti-Serum Amyloid P/SAP antibody [14B4]

• WB

Supplier Data

Western blot - Anti-Serum Amyloid P/SAP antibody [14B4] (AB27313)

In each well of a Novex Wedge well 4-20% Tris-Glycine gel, 0.2 μg of human serum amyloid P component was loaded in a volume of 10 μL of either non-reducing or reducing loading buffer.

A nitrocellulose membrane was used for blotting. The membrane was blocked overnight in 50 mL of PBS supplemented with 0.1% BSA and 0.1% Tween 20.

The nitrocellulose membrane was incubated for 1 h with 20 mL of 2 μg/mL ab27313 Anti Serum Amyloid P component (SAP), human diluted in PBS supplemented with 0.1% BSA and 0.1% Tween 20.

After washing for 3 ×5 min in 50 mL PBS supplemented with 0.1% BSA and 0.1% Tween 20, the membrane was incubated for 1 h with rabbit anti mouse IgG diluted 1 : 2000 and Streptactin-HRP diluted 1 : 2000 in 10 ml of PBS supplemented with 0.1% BSA and 0.1% Tween 20.

After another washing for 3 ×5 min in 50 mL PBS supplemented with 0.1% BSA and 0.1% Tween 20, the membrane was incubated for 5 min in the dark with Clarity Western ECL.

Lanes 1 and 3:

Western blot - Anti-Serum Amyloid P/SAP antibody [14B4] (ab27313)

Lanes 2 and 4:

Western blot - Anti-Serum Amyloid P/SAP antibody [14B4] (ab27313) at 2 µg/mL

Lanes 1 and 3:

molecular weight market

Lane 2:

human serum amyloid P component, unreduced at 0.2 µg

Lane 4:

human serum amyloid P component, reduced at 0.2 µg

Predicted band size: 25 kDa

false

Exposure time: 60s

• ELISA

Supplier Data

ELISA - Anti-Serum Amyloid P/SAP antibody [14B4] (AB27313)

A direct ELISA was performed using SAP as the coating antigen and ab27313 at a concentration of 0.25ug/mL as the capture antibody