AB53130

Anti-Serum Response Factor SRF (phospho S103) antibody

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(5 제품이 사용된 논문 )

Rabbit Polyclonal Serum Response Factor SRF phospho S103 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SRF pS103 aa 50-150.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF (phospho S103) antibody (AB53130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF (phospho S103) antibody (AB53130)

ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

Unknown

Western blot - Anti-Serum Response Factor SRF (phospho S103) antibody (AB53130)

All lanes:

Western blot - Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130) at 1/300 dilution

Lane 1:

LOVO cell extract treated with serum (20%, 15mins)

Lane 2:

LOVO cell extract treated with serum (20%, 15mins) with immunising peptide

Predicted band size: 52 kDa

Observed band size: 55 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SRF pS103 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P11831

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab53130 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Serum Response Factor (SRF) also known as serum response element-binding protein is a transcription factor with a mass of around 67 kDa. SRF regulates gene expression by binding to the serum response element (SRE) found in the promoter region of target genes. It is widely expressed across multiple tissues including muscle brain and epithelial cells. SRF plays a critical role in various cellular functions due to its ability to control the transcription of immediate-early genes in response to a range of extracellular signals.

Biological function summary

SRF influences cellular processes such as growth differentiation and migration. SRF often functions as a part of a transcriptional complex interacting with co-factors like the ternary complex factor (TCF) from the ETS domain family of transcription factors. This interaction modulates the expression of genes involved in cytoskeletal organization and cell cycle regulation. By doing so SRF coordinates complex cellular activities that are essential for normal development and response to environmental changes.

Pathways

SRF plays a significant role in the mitogen-activated protein kinase (MAPK) pathway and the RhoA pathway. In the MAPK pathway SRF acts downstream to mediate the response of cells to growth stimuli working closely with MAPK-regulated kinases. Through the RhoA pathway SRF regulates changes in cytoskeletal dynamics and cell movement with the involvement of proteins such as Rho-associated coiled-coil containing protein kinase (ROCK) and myocardin-related transcription factors (MRTFs).

SRF has a strong connection to cardiovascular diseases and certain types of cancer. Alterations in SRF expression or function can contribute to the development of atherosclerosis and myocardial hypertrophy. Additionally dysregulation in SRF activity has been observed in some cancers where it associates with aberrant cell proliferation and migration. In these contexts proteins such as connective tissue growth factor (CTGF) can interact with SRF further impacting disease progression and severity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.

See full target information SRF pS103

대체 명칭 보기

Serum response factor, SRF

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta physiologica (Oxford, England) 220:263-274 PubMed27739650

2016

Striated muscle activator of Rho signalling (STARS) is reduced in ageing human skeletal muscle and targeted by miR-628-5p.

Applications

Unspecified application

Species

Unspecified reactive species

A P Russell,M A Wallace,M Kalanon,E Zacharewicz,P A Della Gatta,A Garnham,S Lamon

Nature neuroscience 17:1720-7 PubMed25347353

2014

Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A Heller,Hannah M Cates,Catherine J Peña,Haosheng Sun,Ningyi Shao,Jian Feng,Sam A Golden,James P Herman,Jessica J Walsh,Michelle Mazei-Robison,Deveroux Ferguson,Scott Knight,Mark A Gerber,Christian Nievera,Ming-Hu Han,Scott J Russo,Carol S Tamminga,Rachael L Neve,Li Shen,H Steve Zhang,Feng Zhang,Eric J Nestler

The Journal of physiology 591:3749-63 PubMed23753523

2013

Effect of resistance exercise contraction mode and protein supplementation on members of the STARS signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kristian Vissing,Stine K Rahbek,Severine Lamon,Jean Farup,Renae J Stefanetti,Marita A Wallace,Mikkel H Vendelbo,Aaron Russell

Proceedings of the National Academy of Sciences of the United States of America 106:316-21 PubMed19116276

2009

Synaptic activity-responsive element in the Arc/Arg3.1 promoter essential for synapse-to-nucleus signaling in activated neurons.

Applications

EMSA

Species

Unspecified reactive species

Takashi Kawashima,Hiroyuki Okuno,Mio Nonaka,Aki Adachi-Morishima,Nan Kyo,Michiko Okamura,Sayaka Takemoto-Kimura,Paul F Worley,Haruhiko Bito

American journal of physiology. Cell physiology 296:C711-23 PubMed19036941

2008

Embryonic stem cell differentiation into smooth muscle cells is mediated by Nox4-produced H2O2.

Applications

Unspecified application

Species

Unspecified reactive species

Qingzhong Xiao,Zhenling Luo,Anna Elena Pepe,Andriani Margariti,Lingfang Zeng,Qingbo Xu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Serum Response Factor SRF (phospho S103) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF (phospho S103) antibody (AB53130)

Western blot - Anti-Serum Response Factor SRF (phospho S103) antibody (AB53130)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise