AB67156

Anti-SESN1 antibody

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(3 제품이 사용된 논문 )

Mouse Polyclonal SESN1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SESN1.

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-SESN1 antibody (AB67156)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SESN1 antibody (AB67156)

Immunofluorescence of ab67156 at 10 μg/ml on HeLa cells

주요 정보

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SESN1.

Q9Y6P5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p>Requires purification with Protein A prior to ICC/IF.</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

pH: 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SESN1 also known as Sestrin-1 is a protein that has significant significance in cellular stress responses. It has a molecular weight of approximately 57 kDa. This protein mainly expresses in tissues such as liver skin and heart. Sestrin-1 acts as a regulator of cellular homeostasis modulating factors tied to oxidative stress correction. The protein carries antioxidant properties mediating the regeneration of peroxiredoxins which are important for detoxifying peroxides within cells.

Biological function summary

The Sestrin-1 protein plays a role in the cellular defense against oxidative damage. It incorporates into complexes that participate in inhibiting the activity of the mechanistic target of rapamycin complex 1 (mTORC1) a critical regulator of cell growth and metabolism. Sestrin-1 achieves this by activating AMP-activated protein kinase (AMPK) which serves as an energy sensor in the cell. Additionally it can suppress free radical accumulation during periods of cellular stress helping to prevent damage to DNA and proteins.

Pathways

Sestrin-1 is a vital component of the signaling pathways that involve the AMPK and mTOR pathways. It interacts actively with proteins such as TSC2 in mTOR signaling and LKB1 in the AMPK pathway. Through these pathways Sestrin-1 influences the regulation of protein synthesis and autophagy. It acts as a link between energy status and cellular growth control underpinning its involvement in adaptive cellular responses to environmental stresses.

Sestrin-1 involvement connects to conditions like cancer and neurodegenerative diseases. In cancer its role in inhibiting the mTORC1 pathway gives it potential anti-tumorigenic properties as overactive mTOR signaling can lead to uncontrolled cell proliferation. Additionally in neurodegenerative disorders Sestrin-1 reduces stress-induced neuronal damage through its antioxidant functions. Sestrin-1 interacts with proteins like p53 a well-known tumor suppressor to aid in cellular protective mechanisms providing a link between its protective role and disease suppression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed : 25263562, PubMed : 26449471). This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed : 23274085). Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed : 15105503). However, this could not be confirmed (By similarity).

See full target information SESN1

대체 명칭 보기

PA26, SEST1, SESN1, Sestrin-1, p53-regulated protein PA26

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 10:695 PubMed31541080

2019

The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Chollat-Namy,Thouraya Ben Safta-Saadoun,Djazia Haferssas,Guillaume Meurice,Salem Chouaib,Jerome Thiery

Cell discovery 3:17022 PubMed28698800

2017

Metformin-induced ablation of microRNA 21-5p releases Sestrin-1 and CAB39L antitumoral activities.

Applications

Species

Human

Claudio Pulito,Federica Mori,Andrea Sacconi,Frauke Goeman,Maria Ferraiuolo,Patrizia Pasanisi,Carlo Campagnoli,Franco Berrino,Maurizio Fanciulli,Rebecca J Ford,Massimo Levrero,Natalia Pediconi,Ludovica Ciuffreda,Michele Milella,Gregory R Steinberg,Mario Cioce,Paola Muti,Sabrina Strano,Giovanni Blandino

Autophagy 11:2275-87 PubMed26565591

2015

Constitutive autophagy contributes to resistance to TP53-mediated apoptosis in Epstein-Barr virus-positive latency III B-cell lymphoproliferations.

Applications

Unspecified application

Species

Unspecified reactive species

Anaïs Pujals,Loëtitia Favre,Catherine Pioche-Durieu,Aude Robert,Guillaume Meurice,Marion Le Gentil,Sonia Chelouah,Nadine Martin-Garcia,Eric Le Cam,Catherine Guettier,Martine Raphaël,Lyubomir T Vassilev,Philippe Gaulard,Patrice Codogno,Marc Lipinski,Joëlle Wiels

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Anti-SESN1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SESN1 antibody (AB67156)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise