AB97792

Anti-SESN3 antibody

Name: Anti-SESN3 antibody
Brand: Abcam Limited
SKU: AB97792
Rating: 2 (1 reviews)

(1 리뷰)

(9 제품이 사용된 논문 )

Rabbit Polyclonal SESN3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SESN3 aa 1-300.

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-SESN3 antibody (AB97792)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SESN3 antibody (AB97792)

Immunofluorescence analysis of paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cell labeling SESN3 with ab97792 at a 1/200 dilution. The lower image was merged with DNA probe.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SESN3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P58005

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SESN3 also known as Sestrin 3 is a protein involved in cellular stress response with a mass of approximately 57 kDa. This protein works as an antioxidant and helps in regulating the cellular redox state. SESN3 is expressed in various tissues most prominently in metabolically active tissues such as the liver and muscle. It responds to oxidative stress by influencing antioxidant defense mechanisms and also maintains cellular homeostasis.

Biological function summary

SESN3 plays an important role in metabolic regulation and cellular protection. It activates AMP-activated protein kinase (AMPK) contributing to the suppression of the mammalian target of rapamycin complex 1 (mTORC1) signaling. SESN3 does not exist as a part of a larger protein complex itself but interacts with other proteins to exert its functions. Through these interactions SESN3 helps manage cellular metabolism preventing damage from oxidative stress.

Pathways

SESN3 participates in the PI3K/AKT/mTOR and AMPK signaling pathways both essential for regulating cell growth and energy metabolism. It interacts with proteins such as mTOR and TSC2 (tuberous sclerosis complex 2) which are necessary for balancing cellular growth and nutrient availability. SESN3 modulates these pathways by inhibiting mTORC1 activity impacting processes like protein synthesis and autophagy.

SESN3 has associations with metabolic disorders and cancer. Dysregulation of SESN3 affects pathways like AMPK and mTOR linked to type 2 diabetes and obesity. Additionally changes in SESN3 levels influence tumor growth as SESN3 affects processes involved in cell survival and proliferation. SESN3's interaction with AMPK and mTOR links it to cancerous processes highlighting its potential as a therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May function as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway (PubMed : 25263562). May also regulate the insulin-receptor signaling pathway through activation of TORC2 (By similarity). This metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity).

See full target information SESN3

대체 명칭 보기

SEST3, SESN3, Sestrin-3

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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of experimental & clinical cancer research 39:169 PubMed32847606

2020

CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Lili Yin,Xiaofei Sun

Nature communications 11:190 PubMed31929512

2020

Sestrins are evolutionarily conserved mediators of exercise benefits.

Applications

Unspecified application

Species

Unspecified reactive species

Myungjin Kim,Alyson Sujkowski,Sim Namkoong,Bondong Gu,Tyler Cobb,Boyoung Kim,Allison H Kowalsky,Chun-Seok Cho,Ian Semple,Seung-Hyun Ro,Carol Davis,Susan V Brooks,Michael Karin,Robert J Wessells,Jun Hee Lee

Oncogene 39:1080-1097 PubMed31591479

2019

A drug library screen identifies Carbenoxolone as novel FOXO inhibitor that overcomes FOXO3-mediated chemoprotection in high-stage neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Salcher,Gilles Spoden,Judith Hagenbuchner,Sebastian Führer,Teresa Kaserer,Martin Tollinger,Petra Huber-Cantonati,Thomas Gruber,Daniela Schuster,Ronald Gust,Heinz Zwierzina,Thomas Müller,Ursula Kiechl-Kohlendorfer,Michael J Ausserlechner,Petra Obexer

American journal of physiology. Heart and circulat 317:H39-H48 PubMed31026186

2019

Cardioprotective roles of sestrin 1 and sestrin 2 against doxorubicin cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiting Li,Yin Huang,Ian Semple,Myungjin Kim,Zunjian Zhang,Jun Hee Lee

Experimental gerontology 110:23-34 PubMed29751091

2018

Sestrins are differentially expressed with age in the skeletal muscle of men: A cross-sectional analysis.

Applications

Species

Unspecified reactive species

Nina Zeng,Randall F D'Souza,Cameron J Mitchell,David Cameron-Smith

Physiological reports 5: PubMed29263116

2017

Acute resistance exercise induces Sestrin2 phosphorylation and p62 dephosphorylation in human skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Zeng,Randall F D'Souza,Vandre C Figueiredo,James F Markworth,Llion A Roberts,Jonathan M Peake,Cameron J Mitchell,David Cameron-Smith

Oncotarget 8:90132-90143 PubMed29163816

2017

Sestrin2 and sestrin3 suppress NK-92 cell-mediated cytotoxic activity on ovarian cancer cells through AMPK and mTORC1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejin Wang,Weifeng Liu,Deyi Zhuang,Shaoxian Hong,Jingfang Chen

Nature communications 5:4233 PubMed24947615

2014

Hepatoprotective role of Sestrin2 against chronic ER stress.

Applications

Unspecified application

Species

Unspecified reactive species

Hwan-Woo Park,Haeli Park,Seung-Hyun Ro,Insook Jang,Ian A Semple,David N Kim,Myungjin Kim,Myeongjin Nam,Deqiang Zhang,Lei Yin,Jun Hee Lee

American journal of physiology. Gastrointestinal a 307:G58-65 PubMed24833709

2014

The inhibitory effect of ethanol on Sestrin3 in the pathogenesis of ethanol-induced liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xinqin Kang,Kateryna Petyaykina,Rongya Tao,Xiwen Xiong,X Charlie Dong,Suthat Liangpunsakul

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SESN3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SESN3 antibody (AB97792)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (9)

Product promise