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AB96683

Anti-SF3B3 antibody

4

(1 Review)

|

(6 Publications)

Rabbit Polyclonal SF3B3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SF3B3 aa 1150 to C-terminus.

대체 명칭 보기

KIAA0017, SAP130, SF3B3, Splicing factor 3B subunit 3, Pre-mRNA-splicing factor SF3b 130 kDa subunit, STAF130, Spliceosome-associated protein 130, SF3b130, SAP 130

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B3 antibody (AB96683)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B3 antibody (AB96683)

ab96683, at 1/100 dilution, staining SF3B3 in paraffin-embedded Saos-2 by Immunohistochemistry.

Western blot - Anti-SF3B3 antibody (AB96683)
  • WB

CiteAb

Western blot - Anti-SF3B3 antibody (AB96683)

SF3B3 western blot using anti-SF3B3 antibody ab96683. Publication image and figure legend from Chakraborty, P., Huang, J. T. J., et al., 2018, Nat Commun, PubMed 30341290.

ab96683 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab96683 please see the product overview.

DHX9 helicase activity is required for R-loop formation and growth inhibition. a Expression of wild-type DHX9, but not a helicase-defective mutant (pGFP-DHX9dead), promotes R-loop formation in SFPQ-defective cells. Fluorescence intensity of S9.6 staining was measured for n > 50 cells. Statistical significance was determined using Mann–Whitney test (****p < 0.0001). b Expression of wild-type DHX9 but not a helicase-defective mutant confers impaired cell proliferation in SFPQ-depleted cells (siSFPQ8). Cell number was measured using a Casey Cell Counter 48 h after transfection with siRNA (time 0) and again after 3, 5, and 7 days. c RNA Pol II S2P was immunoprecipitated from Hela cell nuclear extracts and co-purification of different splicing factors was probed by western blot (as indicated). Duplicate samples were treated with RNaseA as indicated to demonstrate the requirement of RNA in these interactions

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SF3B3 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15393

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대체품

Primary Antibodies

AB209402

Anti-SF3B3 antibody [EPR18440]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-SF3B3 antibody [EPR18440] (AB209402)

  • 0
  • 0
Primary Antibodies

AB209403

Anti-SF3B3 antibody [EPR18441]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B3 antibody [EPR18441] (AB209403)

  • 0
  • 0

보완 제품

Primary Antibodies

AB56800

Anti-SF3B2 antibody [5D2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B2 antibody [5D2] (AB56800)

  • 0
  • 1
Primary Antibodies

AB70454

Anti-DHX15/prp43 antibody

Immunoprecipitation - Anti-DHX15/prp43 antibody (AB70454)

  • 0
  • 0
Primary Antibodies

AB172614

Anti-U2AF35/U2AF1 antibody [EPR12649(B)]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (AB172614)

  • 5
  • 1
Primary Antibodies

AB248731

Anti-Tat-SF1 antibody [EPR9105(B)] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Tat-SF1 antibody [EPR9105(B)] - BSA and Azide free (AB248731)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SF3B3 also known as SAP130 is a component of the splicing factor 3b complex vital for pre-mRNA processing. It is approximately 130 kDa in mass and prominently expressed across various tissue types including the liver lungs and heart. SF3B3 functions in the spliceosome a complex machinery essential for the removal of introns from pre-mRNA transcripts.
Biological function summary

SF3B3 participates in the assembly and stabilization of the functional spliceosomal complex. It is a part of the larger SF3b complex which is critical for recognizing the branch point sequence in RNA during splicing. This recognition influences the selection of splice sites and impacts the regulation of alternative splicing events. The SF3b complex works with the U2 small nuclear ribonucleoprotein (snRNP) during the splicing process to ensure accurate and efficient mRNA maturation.

Pathways

SF3B3 plays a significant role in the splicing pathway and impacts gene expression regulation through alternative splicing. It works alongside proteins such as SF3B1 and other splicing factors maintaining proper cellular function by influencing transcript variants. Another important pathway involving SF3B3 is RNA transport where it assists in the export of mature and correctly processed mRNA from the nucleus to the cytoplasm.

SF3B3 is closely linked to myelodysplastic syndromes and certain types of cancer such as breast cancer. Mutations or dysregulation of SF3B3 can result in aberrant splicing patterns contributing to tumorigenesis and disease progression. The protein is often studied along with SF3B1 as both are part of the splicing machinery and have been implicated in splicing-related genetic abnormalities that drive these disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 10490618, PubMed : 10882114, PubMed : 12234937, PubMed : 27720643, PubMed : 28781166, PubMed : 32494006, PubMed : 34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 12234937, PubMed : 32494006, PubMed : 34822310). Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed : 12234937, PubMed : 27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed : 12234937). May also be involved in the assembly of the 'E' complex (PubMed : 10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077, PubMed : 33509932).
See full target information SF3B3
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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta pharmaceutica Sinica. B 11:1227-1245 PubMed34094830

2021

Multi-omics approaches identify and as candidate autophagic regulators and druggable targets in invasive breast carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shouyue Zhang,Jin Zhang,Yang An,Xiaoxi Zeng,Ziyi Qin,Yuqian Zhao,Heng Xu,Bo Liu

Nature communications 9:4346 PubMed30341290

2018

DHX9 helicase promotes R-loop formation in cells with impaired RNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Prasun Chakraborty,Jeffrey T J Huang,Kevin Hiom

Clinical cancer research : an official journal of the American Association for Cancer Research 23:3428-3441 PubMed27879367

2016

Alternative Splicing of EZH2 pre-mRNA by SF3B3 Contributes to the Tumorigenic Potential of Renal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Chen,Haibing Xiao,Jin Zeng,Gan Yu,Hui Zhou,Chunhua Huang,Weimin Yao,Wei Xiao,Junhui Hu,Wei Guan,Lily Wu,Jiaoti Huang,Qihong Huang,Hua Xu,Zhangqun Ye

Nucleic acids research 44:8933-8950 PubMed27317697

2016

The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators.

Applications

WB

Species

Mouse

Miriam Llorian,Clare Gooding,Nicolas Bellora,Martina Hallegger,Adrian Buckroyd,Xiao Wang,Dipen Rajgor,Melis Kayikci,Jack Feltham,Jernej Ule,Eduardo Eyras,Christopher W J Smith

The Journal of experimental medicine 210:241-56 PubMed23359068

2013

Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies.

Applications

ICC/IF

Species

Unspecified reactive species

Guang Yang,T Matt Holl,Yang Liu,Yi Li,Xiaozhi Lu,Nathan I Nicely,Thomas B Kepler,S Munir Alam,Hua-Xin Liao,Derek W Cain,Leonard Spicer,John L VandeBerg,Barton F Haynes,Garnett Kelsoe

The Journal of biological chemistry 288:355-67 PubMed23148228

2012

Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling.

Applications

WB

Species

Unspecified reactive species

Yaping Lin-Moshier,Peter J Sebastian,Leeann Higgins,Natalie D Sampson,Jane E Hewitt,Jonathan S Marchant
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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