AB99357
Rabbit Polyclonal SFPQ antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SFPQ aa 650-750.
2 이미지
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody (AB99357)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling SFPQ with ab99357 at 1/500 (0.4 µg/ml). Detection : DAB.
- IP
Unknown
Immunoprecipitation - Anti-SFPQ antibody (AB99357)
ab99357 at 1 μg/ml detecting SFPQ in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 1 second exposure.
For immunoprecipitation, ab99357 was used at at 3 μg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1; IP using ab99357
Lane 2; IP using control IgG.
All lanes:
Immunoprecipitation - Anti-SFPQ antibody (ab99357)
Predicted band size: 76 kDa
false
대체품
![Immunocytochemistry/ Immunofluorescence - Anti-SFPQ antibody [EPR11846] (AB177936)](https://content.abcam.com/products/images/sfpq-antibody-epr11846-ab177936--immunocytochemistry-immunofluorescence-img17998.jpg)
Primary Antibodies
AB246358
Anti-SFPQ antibody [EPR11847] - BSA and Azide free
BOND RX™ Validated|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (AB246358)](https://content.abcam.com/products/images/sfpq-antibody-epr11847-bsa-and-azide-free-ab246358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174150.jpg)
- 0
- 0
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SFPQ antibody [EPR11847] (AB206742)](https://content.abcam.com/products/images/alexa-fluor-488-sfpq-antibody-epr11847-ab206742--immunocytochemistry-immunofluorescence-img134128.jpg)
Primary Antibodies
AB177149
20ul selling size|BOND RX™ Validated|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (AB177149)](https://content.abcam.com/products/images/sfpq-antibody-epr11847-ab177149--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174150.jpg)
- 5
- 3
보완 제품
Primary Antibodies
AB133574
Anti-nmt55 / p54nrb antibody [EPR5270]
RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody [EPR5270] (AB133574)](https://content.abcam.com/products/images/nmt55-p54nrb-antibody-epr5270-ab133574--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135545.jpg)
- 0
- 0
Primary Antibodies
AB259335
Anti-FMRP antibody [EPR23852-90]
BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb
![Immunoprecipitation - Anti-FMRP antibody [EPR23852-90] (AB259335)](https://content.abcam.com/products/images/fmrp-antibody-epr23852-90-ab259335--immunoprecipitation-img11926.jpg)
- 0
- 0
Primary Antibodies
AB16667
BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)](https://content.abcam.com/products/images/ki67-antibody-sp6-ab16667--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417456.jpg)
- 5
- 133
Reactivity 정보
{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
특성 및 보관 정보
제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab99357 was affinity purified using an epitope specific to SFPQ immobilized on solid support.
보관 버퍼
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
SFPQ also known as splicing factor proline and glutamine-rich (PSF) is a multifunctional protein serving in various cellular processes. The protein weighs approximately 76 kDa and is ubiquitously expressed across different tissues. It mainly localizes in the nucleus where it interacts with RNA and other proteins. SFPQ plays essential roles in RNA splicing and transcription regulation among others.
Biological function summary
SFPQ takes part in multiple cellular processes including RNA processing gene expression regulation and DNA repair. SFPQ forms complexes with other proteins such as NONO which enhances its ability in transcriptional control and RNA splicing. By binding to DNA and RNA SFPQ acts as a bridge that connects various molecular events important for cell function and survival.
Pathways
The protein is actively involved in the modulation of RNA metabolic pathways and signal transduction. In particular SFPQ participates in the regulation of alternative splicing processes which can influence the diversity of the proteome. Functionally related proteins like FUS show interactions with SFPQ within these pathways jointly affecting gene expression dynamics.
Aberrations in SFPQ expression or function associate with neurodegenerative diseases and cancers. Mislocalization of SFPQ and its altered expression have been observed in conditions like amyotrophic lateral sclerosis (ALS) and certain leukemia types. In these disorders proteins like TDP-43 have shown associations with SFPQ contributing to the pathology through disrupted RNA processing and cellular stress responses.
제품 프로토콜
For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보
DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer binds DNA (PubMed : 25765647). The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Functions as a transcriptional activator (PubMed : 25765647). Transcriptional repression is mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as a transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF1-stimulated transcriptional activity. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation (By similarity). Required for the assembly of nuclear speckles (PubMed : 25765647). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed : 28712728).
대체 명칭 보기
PSF, SFPQ, 100 kDa DNA-pairing protein, Polypyrimidine tract-binding protein-associated-splicing factor, hPOMp100, PTB-associated-splicing factor
제품이 사용된 논문 (1)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Virology 435:329-40 PubMed23158102
2012
HIV-1 pre-mRNA commitment to Rev mediated export through PSF and Matrin 3.
Applications
Unspecified application
Species
Unspecified reactive species
Anna Kula,Lavina Gharu,Alessandro Marcello
Product promise
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