AB321788

Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free

Advanced Validation
BOND RX™ Validated
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal SGLT1 antibody. Carrier free. Suitable for IHC-P, mIHC and reacts with Transfected cell line - Human, Human, Rat samples.

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Multiplex immunohistochemistry - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

mIHC

Lab

Multiplex immunohistochemistry - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

This data was developed using ab321787, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human small intestine staining SGLT1 with ab321787 at a 1/2000 dilution, ab272692 anti-MUS2 used at 1/2000 dilution and ab252935 anti-EPHB2 used at a 1/500 dilution.

Panel A : merged staining of anti-SGLT1 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-EPHB2 (gray; Opal™570) on human small intestine.

Panel B : anti-SGLT2 staining apical side of the villi in human small intestine.

Panel C : anti-MUC2 staining goblet cells in human small intestine.

Panel D : anti-EPHB2 staining stem cells in human small intestine.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab321787, ab272692 and ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

This data was developed using ab321787, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SGLT1 with ab321787 at 1/2000 (0.268 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human skeletal muscle.
The section was incubated with ab321787 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
We don't expect this antibody to cross-react with SGLT3 (SLC5A4) based on the negative staining observed in skeletal muscle which expresses SGLT3 (PMID : 33385407).

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

This data was developed using ab321787, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a SGLT1 (SLC5A1) expression vector containing a his tag. (B) HEK-293T cells transfected with a SGLT2 (SLC5A2) expression vector containing a his tag. (C) HEK-293T cells transfected with empty vector containing a his tag. tissue labeling SGLT1 with ab321787 at 1/2000 (0.268 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T cells transfected with a SGLT1 (SLC5A1) expression vector containing a his tag No staining on (B) HEK-293T cells transfected with a SGLT2 (SLC5A2) expression vector and (C) HEK-293T cells transfected with empty vector containing a his tag.
The section was incubated with ab321787 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

mIHC

Lab

Multiplex immunohistochemistry - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

This data was developed using ab321787, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining CD34 with ab317588 at a 1/2000 dilution, ab321787 anti-SGLT1 used at 1/2000 dilution and ab212197 anti-Renin used at a 1/4000 dilution.

Panel A : merged staining of anti-CD34 (green; Opal™520), anti-SGLT1 (magenta; Opal™690) and anti-Renin (gray; Opal™570) on human kidney.

Panel B : anti-CD34 staining endothelium in human kidney.

Panel C : anti-SGLT1 staining tubules in human kidney.

Panel D : anti-Renin staining juxtaglomerular cells in human kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317588, ab321787 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

This data was developed using ab321787, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SGLT1 with ab321787 at 1/2000 (0.268 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human kidney (PMID : 26121582).
The section was incubated with ab321787 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

This data was developed using ab321787, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling SGLT1 with ab321787 at 1/2000 (0.268 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human duodenum.
The section was incubated with ab321787 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

This data was developed using ab321787, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SGLT1 with ab321787 at 1/2000 (0.268 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human cerebrum.
The section was incubated with ab321787 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

This data was developed using ab321787, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling SGLT1 with ab321787 at 1/2000 (0.268 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on rat skeletal muscle.
The section was incubated with ab321787 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
We don't expect this antibody to cross-react with SGLT3 (SLC5A4) based on the negative staining observed in skeletal muscle which expresses SGLT3 (PMID : 33385407).

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

This data was developed using ab321787, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SGLT1 with ab321787 at 1/2000 (0.268 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on rat cerebrum (PMID : 8163506).
The section was incubated with ab321787 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

This data was developed using ab321787, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling SGLT1 with ab321787 at 1/2000 (0.268 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat kidney.
The section was incubated with ab321787 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

This data was developed using ab321787, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat duodenum tissue labeling SGLT1 with ab321787 at 1/2000 (0.268 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat duodenum (PMID : 8163506).
The section was incubated with ab321787 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26041-107

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat

Applications

IHC-P, mIHC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "" } } }

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제품 세부 정보

ab321788 is the carrier-free version of ab321787.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Electrogenic Na(+)-coupled sugar symporter that actively transports D-glucose or D-galactose at the plasma membrane, with a Na(+) to sugar coupling ratio of 2 : 1. Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump (PubMed : 20980548, PubMed : 34880492, PubMed : 35077764, PubMed : 8563765, PubMed : 37217492). Has a primary role in the transport of dietary monosaccharides from enterocytes to blood. Responsible for the absorption of D-glucose or D-galactose across the apical brush-border membrane of enterocytes, whereas basolateral exit is provided by GLUT2. Additionally, functions as a D-glucose sensor in enteroendocrine cells, triggering the secretion of the incretins GCG and GIP that control food intake and energy homeostasis (By similarity) (PubMed : 8563765). Together with SGLT2, functions in reabsorption of D-glucose from glomerular filtrate, playing a nonredundant role in the S3 segment of the proximal tubules (By similarity). Transports D-glucose into endometrial epithelial cells, controlling glycogen synthesis and nutritional support for the embryo as well as the decidual transformation of endometrium prior to conception (PubMed : 28974690). Acts as a water channel enabling passive water transport across the plasma membrane in response to the osmotic gradient created upon sugar and Na(+) uptake. Has high water conductivity, comparable to aquaporins, and therefore is expected to play an important role in transepithelial water permeability, especially in the small intestine.

See full target information SLC5A1

대체 명칭 보기

NAGT, SGLT1, SLC5A1, Sodium/glucose cotransporter 1, Na(+)/glucose cotransporter 1, High affinity sodium-glucose cotransporter, Solute carrier family 5 member 1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free

Multiplex immunohistochemistry - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

Multiplex immunohistochemistry - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody [EPR26041-107] - BSA and Azide free (AB321788)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise