AB171943

Anti-SGSM1/RUTBC2 antibody [EPR11563]

RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal SGSM1/RUTBC2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

대체 명칭 보기

KIAA1941, RUTBC2, SGSM1, Small G protein signaling modulator 1, RUN and TBC1 domain-containing protein 2

3 이미지

Flow Cytometry (Intracellular) - Anti-SGSM1/RUTBC2 antibody [EPR11563] (AB171943)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SGSM1/RUTBC2 antibody [EPR11563] (AB171943)

Intracellular flow cytometric analysis of permeabilized 293T cellslabelling SGSM1/RUTBC2 with ab171943 at 1/10 (red) or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-SGSM1/RUTBC2 antibody [EPR11563] (AB171943)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SGSM1/RUTBC2 antibody [EPR11563] (AB171943)

Immunofluorescent analysis of 293T cells labeling SGSM1/RUTBC2 with ab171943 at 1/100 (red). DAPI nuclear staining (blue).

Supplier Data

Western blot - Anti-SGSM1/RUTBC2 antibody [EPR11563] (AB171943)

All lanes:

Western blot - Anti-SGSM1/RUTBC2 antibody [EPR11563] (ab171943) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

Human fetal heart tissue lysate at 10 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 5:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 129 kDa

true

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11563

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SGSM1 also known as RUTBC2 serves as a Rab GTPase-activating protein. It has a mass of approximately 109 kDa. This protein interacts with small GTPases of the Rab family which are pivotal in intracellular vesicle trafficking. SGSM1/RUTBC2 catalyzes the hydrolysis of GTP to GDP on Rab proteins. This activity impacts the regulation of vesicular transport processes. SGSM1/RUTBC2 expression is seen in various tissues indicating its functional diversity.

Biological function summary

SGSM1/RUTBC2 modulates vesicular trafficking and membrane dynamics. It is not part of a large complex but acts as a solitary enzyme influencing membrane trafficking by its interaction with Rabs. Specifically it regulates endocytic and exocytic pathways. Rabs act as molecular switches cycling between active GTP-bound and inactive GDP-bound states and SGSM1 helps facilitate this conversion impacting cellular transport and membrane dynamics.

Pathways

SGSM1/RUTBC2 plays a significant role in endocytic recycling and autophagy pathways. It interacts with Rab7 an important player in the late endocytic pathway and Rab32 integral to lysosomal function. Through mediating the activity of these Rabs SGSM1 influences the trafficking and recycling of membrane proteins and can affect a generation of autophagosomes. This regulation ensures correct cargo delivery and recycling maintaining cellular homeostasis.

SGSM1/RUTBC2 has been associated with neurodegenerative conditions and cancer. Alterations in its expression or function could contribute to improper vesicular transport impacting cellular health and homeostasis. In Alzheimer’s disease dysfunctions of Rab activity influenced by SGSM1 have been noted. Additionally its link to Rab7 suggests a potential involvement in cancer progression as Rab7 dysregulation can affect tumor cell migration and metastasis. Understanding SGSM1 interactions provides insights into these pathologies potentially offering therapeutic targets.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Interacts with numerous Rab family members, functioning as Rab effector for some, and as GTPase activator for others. Promotes GTP hydrolysis by RAB34 and RAB36. Probably functions as a GTPase effector with RAB9A and RAB9B; does not stimulate GTP hydrolysis with RAB9A and RAB9B.

See full target information SGSM1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Aging 17:937-959 PubMed40179319

2025

Decreased mitochondrial NAD+ in WRN deficient cells links to dysfunctional proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Sofie Lautrup,Shi-Qi Zhang,Shinichiro Funayama,Lisa Lirussi,Tina Visnovska,Hoi-Hung Cheung,Marc Niere,Yuyao Tian,Hilde Loge Nilsen,Geir Selbæk,Janna Saarela,Yoshiro Maezawa,Koutaro Yokote,Per Nilsson,Wai-Yee Chan,Hisaya Kato,Mathias Ziegler,Vilhelm A Bohr,Evandro F Fang

iScience 27:109358 PubMed38544565

2024

SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Yang,Ying-Yi Luan,Shan Wang,You-Sheng Yan,Yi-Peng Wang,Jue Wu,Yong-Qing Sun,Jing Zhang,Wen-Qi Chen,Yu-Lan Xiang,Ze-Lu Li,Dong-Liang Zhang,Cheng-Hong Yin

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SGSM1/RUTBC2 antibody [EPR11563]

Flow Cytometry (Intracellular) - Anti-SGSM1/RUTBC2 antibody [EPR11563] (AB171943)

Immunocytochemistry/ Immunofluorescence - Anti-SGSM1/RUTBC2 antibody [EPR11563] (AB171943)

Western blot - Anti-SGSM1/RUTBC2 antibody [EPR11563] (AB171943)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise