AB317835

Anti-SH3GL1/Endophilin A2 antibody [K51/1]

BOND RX™ Validated
Recombinant
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Mouse Recombinant Monoclonal SH3G1 antibody. Suitable for WB, IHC-P, IP and reacts with Rat samples.

대체 명칭 보기

Sh3p8, Endophilin-A2, Endophilin-2, SH3 domain protein 2B, SH3 domain-containing GRB2-like protein 1, SH3p8

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SH3GL1/Endophilin A2 with ab317835 at 1/1000 (1.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on rat cardiac muscle.
The section was incubated with ab317835 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SH3GL1/Endophilin A2 with ab317835 at 1/1000 (1.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining on rat cerebrum.
The section was incubated with ab317835 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

SH3GL1/Endophilin A2 was immunoprecipitated from 0.35 mg Rat testis tissue lysate with ab317835 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317835 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat testis tissue lysate
Lane 2 : ab317835 IP in Rat testis tissue lysate
Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab317835 in rat testis tissue lysate

All lanes:

Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835) at 1/30 dilution

All lanes:

Rat testis tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 41s

Supplier Data

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 2:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 3:

L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

Lane 4:

AR42J (rat pancreatic tumor epithelial cell) whole cell lysate at 20 µg

Lane 5:

H9c2(2-1) (rat cardiomyoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 41 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

Low expression : heart and kidney (PMID : 11518713).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 41 kDa,36 kDa

false

Exposure time: 180s

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

K51/1

Isotype

IgG1

Carrier free

Reacts with

Rat

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SH3GL1 also known as Endophilin A2 is a protein that facilitates membrane curvature during endocytosis through its BAR domain. It weighs approximately 42 kDa. Endophilin A2 is widely expressed in various tissues with notable abundance in the brain. It functions by binding to membrane lipids and interacting with other endocytic proteins. This contributes to the formation of vesicles that ferry molecules into cells vital for nutrient uptake and receptor recycling.

Biological function summary

SH3GL1/Endophilin A2 participates in synaptic vesicle endocytosis a process critical for neurotransmission and proper brain function. It works in association with other proteins in a complex such as dynamin to mediate rapid membrane invagination and fission. By assisting in vesicle formation and release at synaptic terminals it plays a role in maintaining synaptic efficacy and neuronal communication.

Pathways

This protein plays a significant role in the clathrin-mediated endocytosis and synaptic vesicle cycle pathways. In these contexts Endophilin A2 interacts with proteins like clathrin and amphiphysin to enable efficient vesicle budding and release. Its activities connect to the recycling of synaptic vesicles essential for the preservation of synaptic transmission and cell signaling.

SH3GL1/Endophilin A2 associates with neurodegenerative diseases like Alzheimer's disease. Dysfunctional endocytosis resulting from its altered expression or activity can impair synaptic function contributing to disease progression. Other disorders involving this protein include certain types of cancer where its interactions with proteins such as c-Cbl may impact cell proliferation and survival influencing tumorigenesis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).

See full target information Sh3gl1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SH3GL1/Endophilin A2 antibody [K51/1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise