AB236092

Anti-SHARPIN antibody

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Rabbit Polyclonal SHARPIN antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SHARPIN aa 50-200.

대체 명칭 보기

SIPL1, PSEC0216, SHARPIN, Sharpin, Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1, hSIPL1

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHARPIN antibody (AB236092)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHARPIN antibody (AB236092)

Paraffin-embedded human prostate cancer tissue stained for SHARPIN using ab236092 at 1/500 dilution in immunohistochemical analysis. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-SHARPIN antibody (AB236092)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHARPIN antibody (AB236092)

4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized PC-3 (human epithelial cell line from cervix adenocarcinoma) cells stained for SHARPIN (green) using ab236092 at 1/166 dilution in ICC/IF. Secondary antibody is Alexa Fluor^® 488-conjugated Goat Anti-Rabbit IgG (H+L).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHARPIN antibody (AB236092)

Paraffin-embedded human skeletal muscle tissue stained for SHARPIN using ab236092 at 1/500 dilution in immunohistochemical analysis. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SHARPIN aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9H0F6

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SHARPIN also known as SHANK-associated RH domain-interacting protein is a multi-functional protein with a molecular mass of approximately 34 kDa. It primarily acts as a ubiquitin-binding protein and is a part of the LUBAC (Linear Ubiquitin Chain Assembly Complex) involved in various cellular signaling pathways. SHARPIN is expressed in a range of tissues but is highly abundant in lymphoid tissues and the brain. Its mechanical actions involve binding and modulating ubiquitin chains which plays a role in regulating protein stability and signaling processes in cells.

Biological function summary

SHARPIN engages in significant cellular functions by being a critical component of the LUBAC complex. This complex is central to the activation of NF-kB signaling by mediating linear ubiquitination of specific substrate proteins influencing inflammatory responses and cell death regulation. In the immune system SHARPIN regulates the TNF receptor signaling and helps maintain homeostasis. Its absence or mutation can lead to dysregulated cell survival and immune responses highlighting its importance in maintaining cellular functions.

Pathways

SHARPIN integrates into the NF-kB and Wnt signaling pathways which are vital for regulating immune responses and cell fate decisions. Within the NF-kB pathway SHARPIN cooperates with other LUBAC components such as HOIP and HOIL-1L to facilitate ubiquitination necessary for pathway activation. Additionally SHARPIN influences the Wnt pathway through interactions with Dishevelled proteins contributing to developmental and stem cell signaling. The coordination of these pathways by SHARPIN highlights its role in cellular communication and signaling.

SHARPIN has connections to conditions such as chronic inflammation and immunodeficiency syndromes. Dysregulation in SHARPIN function can contribute to chronic inflammatory diseases including psoriasis due to improper NF-kB pathway regulation. Its association with HOIP and HOIL-1L through the LUBAC complex also links it to autoinflammatory conditions characterized by immune system imbalances. SHARPIN mutations are implicated in skin and immune disorders reflecting its broad impact on health and disease manifestation.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed : 21455173, PubMed : 21455180, PubMed : 21455181). LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways (PubMed : 21455173, PubMed : 21455180, PubMed : 21455181). Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation (PubMed : 21455173, PubMed : 21455180, PubMed : 21455181). LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex (PubMed : 21455173, PubMed : 21455180, PubMed : 21455181). The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria (PubMed : 28481331). LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin (PubMed : 28481331). The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation (PubMed : 28481331). Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis (PubMed : 23708998).

See full target information SHARPIN

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of oncology 60: PubMed35506454

2022

Visfatin inhibits colon cancer cell apoptosis and decreases chemosensitivity to 5‑FU by promoting the SDF‑1/CXCR4/Akt axis.

Applications

Unspecified application

Species

Unspecified reactive species

Quan Zhao,Yaxin Long,Wen Cheng,Yingguang Huang,Jinyuan Li,Yuejin Li,Xing Li,Xiaodong Guo,Yu Li,Guosan Li,Kunmei Gong,Jian Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SHARPIN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHARPIN antibody (AB236092)

Immunocytochemistry/ Immunofluorescence - Anti-SHARPIN antibody (AB236092)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHARPIN antibody (AB236092)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise