AB243385

Anti-SHC2 antibody

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Rabbit Polyclonal SHC2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SHC2 aa 400-550.

대체 명칭 보기

SCK, SHCB, SHC2, SHC-transforming protein 2, Protein Sck, SHC-transforming protein B, Src homology 2 domain-containing-transforming protein C2, SH2 domain protein C2

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC2 antibody (AB243385)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC2 antibody (AB243385)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for SHC2 with ab243385 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC2 antibody (AB243385)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for SHC2 with ab243385 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC2 antibody (AB243385)

Formalin-fixed, paraffin-embedded human prostate tissue stained for SHC2 with ab243385 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC2 antibody (AB243385)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for SHC2 with ab243385 at a 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SHC2 antibody (AB243385)

All lanes:

Western blot - Anti-SHC2 antibody (ab243385) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (Human brain glioma cell line) cell lysate

Lane 3:

Human plasma (IgG/HSA depleted)

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 62 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SHC2 aa 400-550. The exact immunogen used to generate this antibody is proprietary information.

P98077

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SHC2—also known as SHC transforming protein 2 or Src homology 2 domain-containing—functions as an adaptor protein involved in intracellular signal transduction. It has a molecular mass of approximately 64 kDa. SHC2 mainly gets expressed in the nervous system and tissues where it contributes to cellular differentiation and growth activities. Its expression level may vary depending on the cell type and developmental stage.

Biological function summary

SHC2 plays significant roles in the regulation of signal transduction pathways that control cell growth apoptosis and differentiation. It participates in the formation of complexes that relay signals from activated receptor tyrosine kinases. SHC2 by binding to these receptors through its SH2 domain helps in the recruitment of downstream signaling molecules like GRB2 leading to the activation of signaling cascades.

Pathways

SHC2 is critically involved in the MAPK/ERK signaling pathway which transmits signals from the cell surface to the DNA in the cell nucleus. This pathway's activation ultimately leads to cell growth and differentiation processes. SHC2 interacts with proteins such as GRB2 and SOS fostering signal transduction that is essential for maintaining proper cellular functions.

SHC2 has been associated with neurodegenerative disorders and certain types of cancer. Alterations in its expression or function can affect cell signaling leading to neural developmental disorders or contributing to tumorigenesis. For example its interaction with receptor tyrosine kinases can modulate pathways involved in cell proliferation connecting SHC2 to cancers where these receptors play a role in the disease's progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons (By similarity).

See full target information SHC2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer medicine 12:10738-10754 PubMed36880364

2023

Sulfotransferase 1C2 promotes hepatocellular carcinoma progression by enhancing glycolysis and fatty acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Liya Jiang,Fang Xu,Chenglong Li,Ting Liu,Qianwei Zhao,Yixian Liu,Ying Zhao,Yamei Li,Zhendong Zhang,Xiaolei Tang,Jintao Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SHC2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC2 antibody (AB243385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC2 antibody (AB243385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC2 antibody (AB243385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC2 antibody (AB243385)

Western blot - Anti-SHC2 antibody (AB243385)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise