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AB326640

Anti-SHIP-1 (phospho Y1022) antibody [JJ082-03]

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Rabbit Recombinant Monoclonal SHIP-1 phospho Y1022 antibody. Suitable for WB and reacts with Human samples.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-SHIP-1 (phospho Y1022) antibody [JJ082-03] (AB326640)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHIP-1 (phospho Y1022) antibody [JJ082-03] (AB326640)

ICC/IF staining of SHIP-1 (phospho Y1022) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with antibody at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.

Western blot - Anti-SHIP-1 (phospho Y1022) antibody [JJ082-03] (AB326640)
  • WB

Supplier Data

Western blot - Anti-SHIP-1 (phospho Y1022) antibody [JJ082-03] (AB326640)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Raji whole cell lysates
Lane 2 : Raji treated with pervanadate whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 133 kDa
Observed band size : 145 kDa
Exposure time : 10 seconds

All lanes:

Western blot - Anti-SHIP-1 (phospho Y1022) antibody [JJ082-03] (ab326640) at 1/1000 dilution

Lane 1:

Raji whole cell lysates at 20 µg

Lane 2:

Raji treated with pervanadate whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 133 kDa

Observed band size: 145 kDa

false

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

JJ082-03

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB96402

Anti-SHIP-1 (phospho Y1020) antibody [EPR425]

RabMAb|Recombinant

Western blot - Anti-SHIP-1 (phospho Y1020) antibody [EPR425] (AB96402)

  • 0
  • 0
Primary Antibodies

AB45142

Anti-SHIP-1 antibody [EP378Y]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SHIP-1 antibody [EP378Y] (AB45142)

  • 0
  • 0
Primary Antibodies

AB75809

Anti-Cdc6 (phospho S54) antibody [EPR759Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (AB75809)

  • 0
  • 0
Primary Antibodies

AB138005

Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] (AB138005)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (PubMed : 10764818, PubMed : 8723348, PubMed : 8769125). Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed : 10764818, PubMed : 8769125, PubMed : 9108392). Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity (PubMed : 16682172). Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.
See full target information INPP5D phospho Y1022

대체 명칭 보기

SHIP, SHIP1, Inositol polyphosphate-5-phosphatase D, Inositol polyphosphate-5-phosphatase of 145 kDa, SH2 domain-containing inositol 5'-phosphatase 1, p150Ship, SIP-145, SH2 domain-containing inositol phosphatase 1, SHIP-1, hp51CN
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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