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AB305249

Anti-SIAH1 antibody [EPR27101-45]

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Rabbit Recombinant Monoclonal SIAH1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
2 이미지
Western blot - Anti-SIAH1 antibody [EPR27101-45] (AB305249)
  • WB

Supplier Data

Western blot - Anti-SIAH1 antibody [EPR27101-45] (AB305249)

Blocking and diluting buffer and concentration : 5% NFDM/TBST   Exposure time : 70 seconds

All lanes:

Western blot - Anti-SIAH1 antibody [EPR27101-45] (ab305249) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 μg

Lane 2:

293T transfected with siRNA specifically targeting SIAH1 whole cell lysate, 20 ug

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 31 kDa

false

Exposure time: 70s

Western blot - Anti-SIAH1 antibody [EPR27101-45] (AB305249)
  • WB

Supplier Data

Western blot - Anti-SIAH1 antibody [EPR27101-45] (AB305249)

Blocking and diluting buffer and concentration : 5% NFDM/TBST   Exposure time : 48 seconds

All lanes:

Western blot - Anti-SIAH1 antibody [EPR27101-45] (ab305249) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 20 μg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 31 kDa

false

Exposure time: 48s

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27101-45

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Reagents, Controls & Accessories

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Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

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Primary Antibodies

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Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (AB52866)

  • 5
  • 31
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SIAH1 also known as Seven In Absentia Homolog 1 functions mechanically as a RING-type E3 ubiquitin-protein ligase. This protein weighs approximately 32 kDa and adds ubiquitin to target proteins tagging them for proteasomal degradation. Researchers have found SIAH1 expressed in various tissue types including brain heart and kidney. By regulating protein stability SIAH1 plays a significant role in maintaining cellular protein homeostasis.
Biological function summary

SIAH1 influences cell cycle control and apoptosis by interacting with components of protein complexes. It associates with the ubiquitin-proteasome pathway to mediate degradation of key regulatory proteins. SIAH1 helps in reducing cellular proliferation and enhances apoptotic signaling which are vital processes for maintaining balance between cell survival and death. As part of these processes studies have connected SIAH1 to modulating the fate of proteins involved in cell cycle arrest.

Pathways

Several interactions of SIAH1 contribute to signal transduction and apoptosis. One significant pathway involving SIAH1 is the p53 signaling pathway. SIAH1 regulates the degradation of proteins within this pathway including p21 and cyclin D1 which influence cellular responses to DNA damage. Additionally SIAH1 interacts with β-catenin linking it to the Wnt signaling pathway. This interaction affects the regulation of cell growth and differentiation.

SIAH1 has links to cancer and neurodegenerative diseases. In cancer altered SIAH1 expression affects tumor suppressor activity through its interaction with p53 which can lead to uncontrolled cell growth. Moreover SIAH1 connects to neurodegenerative disorders like Parkinson's disease where abnormal protein degradation contributes to neuronal death. In such contexts researchers observe altered interactions between SIAH1 and proteins like parkin a ubiquitin ligase also implicated in these diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 28546513, PubMed : 32430360, PubMed : 33591310, PubMed : 9334332, PubMed : 9858595). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP (PubMed : 10747903, PubMed : 11146551, PubMed : 11389839, PubMed : 11389840, PubMed : 11483517, PubMed : 11483518, PubMed : 11752454, PubMed : 12072443). Confers constitutive instability to HIPK2 through proteasomal degradation (PubMed : 18536714, PubMed : 33591310). It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF signaling (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Has some overlapping function with SIAH2 (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Induces apoptosis in cooperation with PEG3 (By similarity). Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus (By similarity). GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins (By similarity). Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4 (By similarity). Also part of the Wnt signaling pathway in which it mediates the Wnt-induced ubiquitin-mediated proteasomal degradation of AXIN1 (PubMed : 28546513, PubMed : 32430360).
See full target information SIAH1

대체 명칭 보기

HUMSIAH, SIAH1, E3 ubiquitin-protein ligase SIAH1, RING-type E3 ubiquitin transferase SIAH1, Seven in absentia homolog 1, Siah-1a, Siah-1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

ACS omega 9:7782-7792 PubMed38405482

2024

BIRC6 Modulates the Protein Stability of Axin to Regulate the Growth, Stemness, and Resistance of Renal Cancer Cells via the β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kaihua Zhong,Xiaohong Wang,Heyuan Zhang,Nanhui Chen,Yang Mai,Sipin Dai,Lawei Yang,Dong Chen,Weifeng Zhong
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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