AB249295

Anti-SIGLEC11 antibody [EPR8506(2)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SIGLEC11 antibody. Carrier free. Suitable for WB and reacts with Human samples.

대체 명칭 보기

UNQ9222/PRO28718, SIGLEC11, Sialic acid-binding Ig-like lectin 11, Sialic acid-binding lectin 11, Siglec-11

1 이미지

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Western blot - Anti-SIGLEC11 antibody [EPR8506(2)] - BSA and Azide free (AB249295)

This data was developed using ab157187, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SIGLEC11 antibody [EPR8506(2)] - BSA and Azide free (ab249295) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 75 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8506(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab249295 is the carrier-free version of ab157187.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SIGLEC11 also known as sialic acid-binding Ig-like lectin 11 is an inhibitory receptor protein characterized by its ability to bind sialic acids. It has a mass of approximately 66 kDa. This protein contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that helps transmit inhibitory signals. SIGLEC11 is predominantly expressed in the immune cells including macrophages and dendritic cells within the central nervous system (CNS). It is also found in certain peripheral tissues.

Biological function summary

SIGLEC11 plays a role in modulating immune responses and maintaining immune homeostasis. By binding to sialic acid-containing glycoproteins SIGLEC11 can influence cell-cell interactions and prevent unnecessary activation of immune cells. It does not form part of a larger complex but directly participates by recruiting phosphatases such as SHP-1 or SHP-2. These recruitments help dephosphorylate substrates in turn dampening immune cell activation.

Pathways

SIGLEC11 contributes to the negative regulation of receptor signaling pathways relevant to the immune system. Within the signaling pathways it impacts the downstream signaling cascade that involves Src family kinases. SIGLEC11 shares interactions with other members of the SIGLEC family engaging in pathways that modulate cellular activation and inflammation such as the MAPK pathway. This ensures balanced immune responses to avoid excessive inflammation.

SIGLEC11 expressions show relevance to neurodegenerative diseases and autoimmune disorders. Altered SIGLEC11 activity is associated with Alzheimer's disease where it may dampen the inflammatory response within the CNS providing a protective mechanism. In autoimmune conditions such as systemic lupus erythematosus (SLE) SIGLEC11 may interact with proteins that regulate self-tolerance and immune activation mitigating inappropriate immune responses. Understanding its function helps to reveal insights into disease mechanisms and potential therapeutic targets.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,8-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules.

See full target information SIGLEC11

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIGLEC11 antibody [EPR8506(2)] - BSA and Azide free

Western blot - Anti-SIGLEC11 antibody [EPR8506(2)] - BSA and Azide free (AB249295)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise