Anti-SIRP alpha antibody [SADF10.1]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [SADF10.1] (AB267409)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling SIRP alpha with ab267409 at 127.3ug/ml, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in THP-1 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1 : ab267409 at 127.3μg/ml followed by ab150080 at a 1/200 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

• WB

Lab

Western blot - Anti-SIRP alpha antibody [SADF10.1] (AB267409)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile weight observed is consistent with what has been described in the literature (PMID : 9712903, 12773380).
Negative control : Jurkat (PMID : 17982459).

All lanes:

Western blot - Anti-SIRP alpha antibody [SADF10.1] (ab267409) at 1.273 µg/mL

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg

Lane 3:

SK-MEL-28 (human malignant melanoma), whole cell lysate at 20 µg

Lane 4:

SK-MEL-2 (human malignant melanoma), whole cell lysate at 20 µg

Lane 5:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 55 kDa

false

Exposure time: 3min