AB238421

Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SLAMF6 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

CD352, KALI, UNQ6123/PRO20080, SLAMF6, SLAM family member 6, Activating NK receptor, NK-T-B-antigen, NTB-A

6 이미지

Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling SLAMF6 with ab224201 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224201).

Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (human Burkitt's lymphoma cell line) cells labeling SLAMF6 with ab224201 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in the Ramos cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab224201).

Flow Cyt

Supplier Data

Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling SLAMF6 with ab224201 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224201).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling SLAMF6 with ab224201 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in the Jurkat cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224201).

Supplier Data

Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224201).

SLAMF6 was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab224201 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224201 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Blocking and Diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] (<a href='/ko/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a>) at 1/1000 dilution

Lane 1:

Ramos whole cell lysate 10 μg (Input) at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a> at 1/30 IP in Ramos whole cell lysate (+) at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a> in Ramos whole cell lysate (-) at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 37 kDa

true

Exposure time: 10s

Supplier Data

Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab224201).

SLAMF6 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab224201 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224201 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] (<a href='/ko/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a>) at 1/500 dilution

Lane 1:

Jurkat whole cell lysate (input) at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a> at 1/30 IP in Jurkat whole cell lysate (+) at 10 µg

Lane 3:

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 37 kDa

true

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22170

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab238421 is the carrier-free version of ab224201.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SLAMF6 also known as CD352 is a protein that functions as a signaling lymphocytic activation molecule. It is a member of the SLAM family and is found on the surface of many immune cells including T cells B cells and natural killer (NK) cells. SLAMF6 has a molecular weight of approximately 71 kDa. This protein plays a role in cell-to-cell adhesion and transduction of activation signals. Its expression appears prominently in lymphoid tissues directing immune responses.

Biological function summary

SLAMF6 assists in modulating immune cell interactions and is part of specific receptor complexes. It influences the activation and proliferation of immune cells and contributes to the maintenance of immune homeostasis. SLAMF6 interacts with signaling pathways involving SAP (SLAM-associated protein) and EAT-2 which facilitate downstream signaling events essential for immune cell function and communication.

Pathways

SLAMF6 integrates within the SLAM receptor signaling pathway impacting the regulation of immune responses. It plays a role in the NF-kB signaling pathway important for inflammatory and immune responses. Related proteins in these pathways include SAP and EAT-2 important in signal transduction cascades that affect cell survival differentiation and function of immune cells.

SLAMF6 ties to autoimmune diseases and certain cancers. It is involved in autoimmune conditions such as systemic lupus erythematosus where malfunction in immune regulation occurs. Additionally deregulation of SLAMF6 expression associates with lymphomas impacting cell communication and immune function. In these conditions SLAMF6 interacts with SAP influencing the disease progression through altered immune signaling pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors (PubMed : 11489943, PubMed : 16920955). Positive signaling in NK cells implicates phosphorylation of VAV1. NK cell activation seems to depend on SH2D1B and not on SH2D1A (PubMed : 16920955). In conjunction with SLAMF1 controls the transition between positive selection and the subsequent expansion and differentiation of the thymocytic natural killer T (NKT) cell lineage (By similarity). Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A (PubMed : 16920955, PubMed : 22184727). Promotes recruitment of RORC to the IL-17 promoter (PubMed : 22989874). In conjunction with SLAMF1 and CD84/SLAMF5 may be a negative regulator of the humoral immune response. In the absence of SH2D1A/SAP can transmit negative signals to CD4(+) T-cells and NKT cells. Negatively regulates germinal center formation by inhibiting T-cell : B-cell adhesion; the function probably implicates increased association with PTPN6/SHP-1 via ITSMs in absence of SH2D1A/SAP. However, reported to be involved in maintaining B-cell tolerance in germinal centers and in preventing autoimmunity (By similarity).

See full target information SLAMF6

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Odontology 111:630-639 PubMed36460825

2022

Regeneration of sensory nerve branches in extraction socket and surrounding alveolar bone in rat: immunohistochemical observation of the axon and myelin sheath changes.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Kakuta,Satoru Matsunaga,Yuto Otsu,Kei Kitamura,Shinichi Abe,Yasutomo Yajima,Hideshi Sekine

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free

Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

Flow Cytometry - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

Immunocytochemistry/ Immunofluorescence - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] - BSA and Azide free (AB238421)

관련 conjugated 항체와 다양한 조성의 항체 (6)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise