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AB244342

Anti-SLC12A1/NKCC2 antibody

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Rabbit Polyclonal SLC12A1/NKCC2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLC12A1 aa 1-150.

대체 명칭 보기

NKCC2, SLC12A1, Solute carrier family 12 member 1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, Kidney-specific Na-K-Cl symporter, Na-K-2Cl cotransporter 2, BSC1

5 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody (AB244342)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody (AB244342)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for SLC12A1/NKCC2 with ab244342 at a 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SLC12A1/NKCC2 antibody (AB244342)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLC12A1/NKCC2 antibody (AB244342)

PFA fixed, Triton X-100 permeabilized RPTECH TERT1 (Human cell line) cells labeling SLC12A1/NKCC2 using ab244342 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody (AB244342)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody (AB244342)

Formalin-fixed, paraffin-embedded human kidney tissue stained for SLC12A1/NKCC2 with ab244342 at a 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody (AB244342)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody (AB244342)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for SLC12A1/NKCC2 with ab244342 at a 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody (AB244342)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody (AB244342)

Formalin-fixed, paraffin-embedded human liver tissue stained for SLC12A1/NKCC2 with ab244342 at a 1/50 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SLC12A1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q13621

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대체품

Primary Antibodies

AB240542

Anti-SLC12A1/NKCC2 antibody

  • 0
  • 0
Primary Antibodies

AB191315

Anti-SLC12A1/NKCC2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

  • 0
  • 0
Primary Antibodies

AB171747

Anti-SLC12A1/NKCC2 antibody [EPR11842]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SLC12A1/NKCC2 antibody [EPR11842] (AB171747)

  • 0
  • 0

보완 제품

Primary Antibodies

AB199975

Anti-Aquaporin 2 antibody [EPR21080]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-Aquaporin 2 antibody [EPR21080] (AB199975)

  • 5
  • 2
Primary Antibodies

AB95302

Anti-SLC12A3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (AB95302)

  • 5
  • 1
Primary Antibodies

AB76969

Anti-Lrp2 / Megalin antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody (AB76969)

  • 5
  • 24
Primary Antibodies

AB89901

Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (AB89901)

  • 5
  • 43
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The SLC12A1 protein also known as the NKCC2 transporter is an important player in ion transport within renal physiology. With a molecular weight of around 120 kDa NKCC2 functions in the reabsorption of sodium potassium and chloride ions a function facilitated by its location in the thick ascending limb of the loop of Henle in the kidneys. This location and functionality are critical for renal concentration mechanisms and contribute to the maintenance of electrolyte and fluid balance.
Biological function summary

This transporter plays an essential role in concentrating urine and regulating blood pressure. NKCC2 is a part of the larger solute carrier family and does not usually form complexes with other proteins. It facilitates the trans-epithelial movement of Na+ K+ and 2Cl- ions from the lumen into renal epithelial cells which is fundamental for the creation of an osmotic gradient essential for water reabsorption.

Pathways

The SLC12A1 protein is a significant component of renal electrolyte transport and blood pressure regulation pathways. It is intricately involved in the renin-angiotensin-aldosterone system (RAAS) which regulates blood volume and systemic vascular resistance. In these pathways NKCC2's activity tightly connects it to other ion channel proteins and transporters such as ENaC which also play roles in blood pressure regulation by influencing sodium handling in the nephron.

Dysregulation of NKCC2 function can result in Bartter syndrome and hypertension. Bartter syndrome a rare disorder characterized by salt wasting and electrolyte imbalances stems from mutations affecting NKCC2. This transporter also impacts hypertension where its dysregulation can alter sodium reabsorption processes linking it to other proteins involved in blood pressure control such as the mineralocorticoid receptor. Understanding the role of NKCC2 provides insight into potential therapeutic targets for these conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation (PubMed : 21321328). Electrically silent transporter system (By similarity).
See full target information SLC12A1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Circulation. Heart failure 17:e011504 PubMed38910562

2024

Overexpression of in Cardiomyocytes Promotes Cardiac Reverse Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Mengda Xu,Hang Zhang,Yuan Chang,Xiumeng Hua,Xiao Chen,Yixuan Sheng,Dan Shan,Mengni Bao,Shengshou Hu,Jiangping Song
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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