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AB241348

Anti-SLC12A6/KCC3 antibody

3

(1 리뷰)

|

(1 출판물)

Rabbit Polyclonal SLC12A6/KCC3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC12A6 aa 1-100.

대체 명칭 보기

KCC3, SLC12A6, Solute carrier family 12 member 6, Electroneutral potassium-chloride cotransporter 3, K-Cl cotransporter 3

1 이미지
Western blot - Anti-SLC12A6/KCC3 antibody (AB241348)
  • WB

Supplier Data

Western blot - Anti-SLC12A6/KCC3 antibody (AB241348)

Prepared using RIPA buffer.

All lanes:

Western blot - Anti-SLC12A6/KCC3 antibody (ab241348) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 128 kDa

false

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SLC12A6 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UHW9

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대체품

Primary Antibodies

AB191451

Anti-SLC12A6/KCC3 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A6/KCC3 antibody - C-terminal (AB191451)

  • 0
  • 1

보완 제품

Primary Antibodies

AB115607

Anti-KCC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC1 antibody (AB115607)

  • 0
  • 0
Primary Antibodies

AB10530

Anti-Nucleophosmin antibody [FC82291]

Western blot - Anti-Nucleophosmin antibody [FC82291] (AB10530)

  • 5
  • 27
Primary Antibodies

AB73113

Anti-LRSAM1 antibody

Western blot - Anti-LRSAM1 antibody (AB73113)

  • 0
  • 0
Primary Antibodies

AB166630

Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker (AB166630)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
Antibody was affinity purified using an epitope specific to SLC12A6/KCC3 immobilized on solid support
보관 버퍼
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SLC12A6 also known as KCC3 is a potassium-chloride cotransporter involved in regulating ion homeostasis. The protein is big weighing approximately 135 kDa. It moves potassium and chloride ions across cell membranes which affects osmotic balance and cell volume. KCC3 is found widely in the human body with higher expression in the nervous system kidneys and heart. This wide expression suggests its important role in maintaining balance of ions in various tissues.
Biological function summary

KCC3 impacts cellular processes by being an important part of regulating salt balance within cells. It belongs to the cation-chloride cotransporter family and plays an important role in cell volume regulation. KCC3 does not usually function as part of a larger protein complex but acts as a standalone transporter. It helps stabilize cell environment which is necessary for correct cellular signaling and function.

Pathways

KCC3 is integral to maintaining the proper function of the WNK-SPAK/OSR1 kinase pathway. This pathway regulates ion transport and adjusts cell volume after osmotic stress. KCC3 interacts with proteins from this pathway like WNK kinases and SPAK which phosphorylate KCC3 to control its activity. As the transporter modifies ion flow it indirectly affects other signaling pathways dependent on ionic balance within cells.

Mutations in KCC3 link to Hereditary Motor and Sensory Neuropathy with Agenesis of the Corpus Callosum (HMSN/ACC). This disorder affects nerve function indicating KCC3’s role in nervous system health. Additionally KCC3 associates with epilepsy where imbalanced ion homeostasis disrupts neuron function. Related proteins such as WNK kinases when dysregulated have implications for these conditions as well highlighting the complex network KCC3 is involved in.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Isoform 1. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 10600773, PubMed : 11551954, PubMed : 16048901, PubMed : 18566107, PubMed : 19665974, PubMed : 21628467, PubMed : 27485015). May contribute to cell volume homeostasis in single cells (PubMed : 16048901, PubMed : 27485015).. Isoform 2. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 16048901, PubMed : 33199848, PubMed : 34031912). May contribute to cell volume homeostasis in single cells (Probable).. Isoform 3. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 16048901). May contribute to cell volume homeostasis in single cells (Probable).. Isoform 4. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 16048901). May contribute to cell volume homeostasis in single cells (Probable).. Isoform 5. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 16048901). May contribute to cell volume homeostasis in single cells (Probable).. Isoform 6. Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 16048901). May contribute to cell volume homeostasis in single cells (Probable).
See full target information SLC12A6
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Brain communications 4:fcac102 PubMed35602653

2022

Disruption of cellular iron homeostasis by missense variants causes severe neurodevelopmental delay, dystonia and seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Nunziata Maio,Russell P Saneto,Richard Steet,Marcio A Sotero de Menezes,Cindy Skinner,Tracey A Rouault
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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