Anti-SLC25A38 antibody [EPBHMR1]

• WB

Lab

Western blot - Anti-SLC25A38 antibody [EPBHMR1] (AB133614)

Western blot : Anti-SLC25A38 antibody [EPBHMR1] ab133614 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 33 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SLC25A38 antibody [EPBHMR1] (ab133614) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysates at 20 µg

Lane 2:

Western blot - Human SLC25A38 knockout HEK-293T cell line (<a href='/ko/products/cell-lines/human-slc25a38-knockout-hek-293t-cell-line-ab266194'>ab266194</a>) at 20 µg

Lane 3:

Jurkat cell lysates at 20 µg

Lane 4:

Daudi cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

• WB

Unknown

Western blot - Anti-SLC25A38 antibody [EPBHMR1] (AB133614)

All lanes:

Western blot - Anti-SLC25A38 antibody [EPBHMR1] (ab133614) at 1/1000 dilution

All lanes:

TF-1 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false