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AB325660

Anti-Slc32a1 antibody [L118/80] - Chicken IgY (Chimeric)

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Chicken Recombinant Monoclonal SLC32A1/VGAT antibody. Suitable for IHC-P, WB and reacts with Rat, Transfected cell lysate - Rat samples.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc32a1 antibody [L118/80] - Chicken IgY (Chimeric) (AB325660)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc32a1 antibody [L118/80] - Chicken IgY (Chimeric) (AB325660)

Representative FFPE section of rat brain cerebellum showing Slc32a1 staining. The sections were stained with ab325660 using goat anti-chicken IgY FITC secondary antibody (green). The stained sections were mounted with Fluoroshield (DAPI and DABCO) mounting medium. DAPI (blue) stains cell nuclei. The antibody stains the GABAergic terminals and neurons in the cerebellum. Magnification 100X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc32a1 antibody [L118/80] - Chicken IgY (Chimeric) (AB325660)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slc32a1 antibody [L118/80] - Chicken IgY (Chimeric) (AB325660)

Representative FFPE section of rat brain cerebellum showing Slc32a1 staining. The sections were stained with ab325660 using goat anti-chicken IgY FITC secondary antibody (green). The stained sections were mounted with Fluoroshield (DAPI and DABCO) mounting medium. DAPI (blue) stains cell nuclei. The antibody stains the GABAergic terminals and neurons in the cerebellum. Magnification 400X.

Western blot - Anti-Slc32a1 antibody [L118/80] - Chicken IgY (Chimeric) (AB325660)
  • WB

Supplier Data

Western blot - Anti-Slc32a1 antibody [L118/80] - Chicken IgY (Chimeric) (AB325660)

Western blotting of mock transfected and VGAT-FLAG transfected COS-7 lysates with an anti-VGAT mouse mAb and ab325660 (5 µg/mL). The mouse mAb and ab325660 were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. The antibody recognizes exogenous VGAT with similar sensitivity as the anti-VGAT mouse Mab only in VGAT-FLAG transfected COS-7 lysates at 57 kDa. VGAT is an alternative name for Slc32a1.

All lanes:

Western blot - Anti-Slc32a1 antibody [L118/80] - Chicken IgY (Chimeric) (ab325660) at 5 µg/mL

Lanes 1 and 3:

mock transfected COS-7 lysates

Lanes 2 and 4:

VGAT-FLAG transfected COS-7 lysates

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 57 kDa

false

주요 정보

Host species

Chicken

Clonality

Monoclonal

Clone number

L118/80

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>No cross-reactivity reported.</p>

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보완 제품

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  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L118/80 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L118/80 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
보관 버퍼
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals (By similarity) (PubMed : 9349821). The transport is equally dependent on the chemical and electrical components of the proton gradient (PubMed : 9349821). May also transport beta-alanine (PubMed : 23919636). Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake (By similarity).
See full target information Slc32a1

대체 명칭 보기

Vgat, Viaat, Slc32a1, Vesicular inhibitory amino acid transporter, GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, rat UNC-47 homolog, rGVAT, RUNC-47
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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