AB139683

Anti-SLD5 antibody [EPR9318]

RabMAb
Recombinant
각 태그의 뜻

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(5 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal SLD5 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 5 publications.

대체 명칭 보기

SLD5, GINS4, DNA replication complex GINS protein SLD5, GINS complex subunit 4

2 이미지

Flow Cytometry (Intracellular) - Anti-SLD5 antibody [EPR9318] (AB139683)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SLD5 antibody [EPR9318] (AB139683)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling SLD5 with ab139683 at 1/100 dilution (red). Rabbit IgG negative control (green).

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Western blot - Anti-SLD5 antibody [EPR9318] (AB139683)

All lanes:

Western blot - Anti-SLD5 antibody [EPR9318] (ab139683) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

PC3 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 26 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9318

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SLD5 also known as GINS4 is a subunit of the GINS complex. It has a molecular mass of approximately 22 kDa. SLD5 plays a significant role in DNA replication specifically during the initiation and elongation phases. The protein is expressed in various tissues with higher levels observed in proliferating cells. By binding to DNA replication origins SLD5 ensures the proper unwinding and loading of other replication factors.

Biological function summary

SLD5 interacts with other components of the GINS complex to form a stable structure necessary for the replication fork's progression. The GINS complex comprising SLD5 PSF1 PSF2 and PSF3 couples with other protein assemblies like CDC45 and MCM (mini chromosome maintenance) proteins. This dynamic association enables the replication machinery to effectively duplicate the genome without errors.

Pathways

SLD5 plays a part in the initiation of the DNA replication pathway. It partners with proteins like CDC45 and the MCM complex to form a replicative helicase. This assembly serves to unwound the DNA double helix facilitating replication. Furthermore interactions with ATR and ATM pathways position SLD5 within the larger network of DNA damage response ensuring any replication stress is adequately managed.

SLD5 exhibits connections with cancers such as breast cancer and colorectal cancer. Alterations in SLD5 expression can influence tumor progression by modulating cell proliferation rates. Through these pathways proteins like MCM and CDC45 are also implicated serving as key regulators in oncogenic processes where SLD5 activity expands its influence. Understanding these relationships offers insights into potential therapeutic targets for controlling abnormal cell growth.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PubMed : 17417653, PubMed : 28414293). GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232).

See full target information GINS4

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cells, tissues, organs 213:55-66 PubMed36273455

2022

MicroRNA-133a-3p Inhibits Lung Adenocarcinoma Development and Cisplatin Resistance through Targeting GINS4.

Applications

Unspecified application

Species

Unspecified reactive species

Yafu Zhou,Jianhua Yan,Huiguo Chen,Wenwu Zhou,Jinsong Yang

OncoTargets and therapy 13:11433-11444 PubMed33192076

2020

Expression Profile of GINS Complex Predicts the Prognosis of Pancreatic Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Fanqin Bu,Xiaojian Zhu,Xuan Yi,Chen Luo,Kang Lin,Jinfeng Zhu,Cegui Hu,Zitao Liu,Jiefeng Zhao,Chao Huang,Wenjun Zhang,Jun Huang

PLoS biology 17:e2006767 PubMed30695077

2019

The Cdk8/19-cyclin C transcription regulator functions in genome replication through metazoan Sld7.

Applications

Unspecified application

Species

Unspecified reactive species

Kerstin Köhler,Luis Sanchez-Pulido,Verena Höfer,Anika Marko,Chris P Ponting,Ambrosius P Snijders,Regina Feederle,Aloys Schepers,Dominik Boos

The Journal of clinical investigation 127:1991-2006 PubMed28414293

2017

Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Cottineau,Molly C Kottemann,Francis P Lach,Young-Hoon Kang,Frédéric Vély,Elissa K Deenick,Tomi Lazarov,Laure Gineau,Yi Wang,Andrea Farina,Marie Chansel,Lazaro Lorenzo,Christelle Piperoglou,Cindy S Ma,Patrick Nitschke,Aziz Belkadi,Yuval Itan,Bertrand Boisson,Fabienne Jabot-Hanin,Capucine Picard,Jacinta Bustamante,Céline Eidenschenk,Soraya Boucherit,Nathalie Aladjidi,Didier Lacombe,Pascal Barat,Waseem Qasim,Jane A Hurst,Andrew J Pollard,Holm H Uhlig,Claire Fieschi,Jean Michon,Vladimir P Bermudez,Laurent Abel,Jean-Pierre de Villartay,Frédéric Geissmann,Stuart G Tangye,Jerard Hurwitz,Eric Vivier,Jean-Laurent Casanova,Agata Smogorzewska,Emmanuelle Jouanguy

Nucleic acids research 45:169-180 PubMed27679476

2016

H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication.

Applications

Species

Unspecified reactive species

Rentian Wu,Zhiquan Wang,Honglian Zhang,Haiyun Gan,Zhiguo Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLD5 antibody [EPR9318]

Flow Cytometry (Intracellular) - Anti-SLD5 antibody [EPR9318] (AB139683)

Western blot - Anti-SLD5 antibody [EPR9318] (AB139683)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise