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AB65113

Anti-SLK antibody

4

(1 리뷰)

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(2 제품이 사용된 논문 )

Rabbit Polyclonal SLK antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications.
3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-SLK antibody (AB65113)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLK antibody (AB65113)

Immunofluorescence analysis of HepG2 cells, using ab65113 antibody at 1/500-1/1000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLK antibody (AB65113)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLK antibody (AB65113)

This image shows human skeletal muscle tissue stained with ab65113 at 1/50 - 1/100 dilution; with and without the immunising peptide.

Western blot - Anti-SLK antibody (AB65113)
  • WB

Unknown

Western blot - Anti-SLK antibody (AB65113)

The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

All lanes:

Western blot - Anti-SLK antibody (ab65113) at 1/500 dilution

Lane 1:

extracts from COLO205 cells

Lane 2:

extracts from COLO205 cells with immunising peptide

Predicted band size: 143 kDa

Observed band size: 143 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB226986

Anti-SLK antibody

Immunocytochemistry/ Immunofluorescence - Anti-SLK antibody (AB226986)

  • 0
  • 0

보완 제품

Biochemicals

AB141122

(±)-Palmitoylcarnitine chloride, Protein kinase C (PKC) inhibitor

Functional Studies - (±)-Palmitoylcarnitine chloride, Protein kinase C (PKC) inhibitor (AB141122)

  • 0
  • 0
Primary Antibodies

AB70484

Anti-LOK antibody

Immunoprecipitation - Anti-LOK antibody (AB70484)

  • 0
  • 0
Primary Antibodies

AB37259

Anti-Cathepsin K antibody [3F9]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [3F9] (AB37259)

  • 0
  • 0
Primary Antibodies

AB109544

Anti-VAV3 (phospho Y173) antibody [EPR1068A]

RabMAb|Recombinant

Western blot - Anti-VAV3 (phospho Y173) antibody [EPR1068A] (AB109544)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SLK also known as Ste20-like kinase or STLK3 is a serine/threonine-protein kinase. It shows mechanical activity through phosphorylation of specific substrates facilitating cellular processes like migration and proliferation. SLK has an approximate mass of 140 kDa and predominantly occurs in various tissues including epithelial and neural tissues. Its expression is regulated by multiple factors contributing to its dynamic functional role in cells.
Biological function summary

SLK plays an essential role in regulating cytoskeletal organization and cell motility allowing rearrangement of actin fibers. It operates as part of a complex involved in signal transduction cascades that influence cellular shape and movement. This kinase modulates pathways important for cell cycle progression and apoptosis affecting cellular dynamics and integrity.

Pathways

SLK is integral to both the Rho and MAPK signaling pathways which are fundamental for cellular stress responses and growth signals. Within these pathways SLK interacts with proteins such as MINK1 and TAO1 contributing to the coordination of cellular responses to environmental changes and stress signals. SLK's role supports critical intracellular communications that decide cellular fate and adaptation processes.

SLK associates with cancer particularly in cases involving abnormal cell migration and metastasis. It interacts with proteins like the focal adhesion kinase (FAK) which strengthens its participation in oncogenic pathways. SLK also has links to neurodegenerative diseases where its deregulation affects cell survival and death pathways influencing disease progression due to its involvement with proteins like caspase-3 in apoptotic processes.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Mediates apoptosis and actin stress fiber dissolution.
See full target information SLK

대체 명칭 보기

KIAA0204, STK2, SLK, STE20-like serine/threonine-protein kinase, STE20-like kinase, hSLK, CTCL tumor antigen se20-9, STE20-related serine/threonine-protein kinase, Serine/threonine-protein kinase 2, STE20-related kinase
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Genome research 27:1087-1097 PubMed28420690

2017

Identification of protein features encoded by alternative exons using Exon Ontology.

Applications

ICC/IF

Species

Human

Léon-Charles Tranchevent,Fabien Aubé,Louis Dulaurier,Clara Benoit-Pilven,Amandine Rey,Arnaud Poret,Emilie Chautard,Hussein Mortada,François-Olivier Desmet,Fatima Zahra Chakrama,Maira Alejandra Moreno-Garcia,Evelyne Goillot,Stéphane Janczarski,Franck Mortreux,Cyril F Bourgeois,Didier Auboeuf

Oncogene 29:2393-403 PubMed20154719

2010

ASAP1 promotes tumor cell motility and invasiveness, stimulates metastasis formation in vivo, and correlates with poor survival in colorectal cancer patients.

Applications

WB

Species

Human

T Müller,U Stein,A Poletti,L Garzia,M Rothley,D Plaumann,W Thiele,M Bauer,A Galasso,P Schlag,M Pankratz,M Zollo,J P Sleeman
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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