AB196599

Anti-SLP76 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal SLP76 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LCP2.

대체 명칭 보기

Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76, LCP2

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-SLP76 antibody (AB196599)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLP76 antibody (AB196599)

Immunofluoresence analysis of HeLa cells labeling SLP76 with ab196599 at 1/50 dilution. Blue : DAPI for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody (AB196599)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody (AB196599)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SLP76 with ab196599 at 1/100 dilution.

Supplier Data

Western blot - Anti-SLP76 antibody (AB196599)

All lanes:

Western blot - Anti-SLP76 antibody (ab196599) at 1/500 dilution

Lane 1:

U937 cell extract

Lane 2:

BT-474 cell extract

Lane 3:

Mouse spleen extract

Lane 4:

Mouse liver extract

Predicted band size: 60 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LCP2. The exact immunogen used to generate this antibody is proprietary information.

Q13094

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SLP76 also known as Src homology 2 domain-containing leukocyte phosphoprotein of 76 kDa plays a pivotal role in intracellular signaling. This adaptor protein weighs approximately 76 kilodaltons and is predominantly expressed in hematopoietic cells particularly T cells NK cells mast cells and platelets. SLP76 functions to relay signals from the cell surface to the nucleus affecting cellular responses including activation proliferation and differentiation.

Biological function summary

This adaptor protein serves as a scaffold linking membrane-proximal signaling to downstream cellular effectors. SLP76 forms part of a larger signaling complex with proteins such as LAT (Linker for Activation of T cells) and Gads facilitating the assembly of multi-protein complexes necessary for signal transduction. It coordinates the recruitment and activation of several enzymes and substrates which leads to robust immune cell responses.

Pathways

The function of SLP76 integrates into T cell receptor (TCR) signaling and integrin signaling pathways. In TCR signaling SLP76 interacts closely with proteins LAT and ZAP-70 which are triggered upon antigen recognition. This interaction propagates the immune response by activating downstream effectors like MAP kinases and NF-kB. In integrin signaling SLP76 helps mediate cytoskeletal rearrangements which are important for cell migration and adhesion.

Mutations or dysregulation of SLP76 associate with immunodeficiencies and hematologic malignancies. Aberrant SLP76 activity can lead to inappropriate activation or inactivation of immune responses contributing to disorders as SCID (Severe Combined Immunodeficiency) and some types of leukemia. Additionally it connects with proteins such as LAT in disease contexts reflecting a shared pathway disruption contributing to the pathophysiology of these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells (PubMed : 11313406, PubMed : 33159816). Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK (PubMed : 8673706, PubMed : 8702662). In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation (PubMed : 11313406). Also plays an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses (PubMed : 33436632).

See full target information LCP2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Revista internacional de andrologia 22:50-58 PubMed39748770

2025

By ameliorating redox imbalance, patients of oligoasthenospermia increased sperm acrosin activity after smoking cessation in Chinese.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyuan Lin,Yangkun Feng,Yun Zhang,Qingwen He,Ninghan Feng

Leukemia & lymphoma 60:1697-1708 PubMed30570341

2018

Retinoic acid and 6-formylindolo(3,2-b)carbazole (FICZ) combination therapy reveals putative targets for enhancing response in non-APL AML.

Applications

Unspecified application

Species

Unspecified reactive species

Rodica P Bunaciu,Robert J MacDonald,Holly A Jensen,Feng Gao,Xin Wang,Lynn Johnson,Jeffrey D Varner,Andrew Yen

Molecular cell 72:1021-1034.e4 PubMed30472193

2018

Transcriptome Landscape of Human Folliculogenesis Reveals Oocyte and Granulosa Cell Interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoyao Zhang,Zhiqiang Yan,Qingyuan Qin,Vicki Nisenblat,Hsun-Ming Chang,Yang Yu,Tianren Wang,Cuiling Lu,Ming Yang,Shuo Yang,Ying Yao,Xiaohui Zhu,Xi Xia,Yujiao Dang,Yixin Ren,Peng Yuan,Rong Li,Ping Liu,Hongyan Guo,Jinsong Han,Haojie He,Kun Zhang,Yiting Wang,Yu Wu,Meng Li,Jie Qiao,Jie Yan,Liying Yan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLP76 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SLP76 antibody (AB196599)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody (AB196599)

Western blot - Anti-SLP76 antibody (AB196599)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise