AB227223

Anti-Smad3 antibody - ChIP Grade

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(7 제품이 사용된 논문 )

Rabbit Polyclonal SMAD3 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMAD3.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 antibody - ChIP Grade (AB227223)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 antibody - ChIP Grade (AB227223)

Paraffin-embedded human gastric cancer tissue stained for Smad3 using ab227223 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Smad3 antibody - ChIP Grade (AB227223)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Smad3 antibody - ChIP Grade (AB227223)

PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Smad3 (green) using ab227223 at 1/200 dilution in ICC/IF.

Nuclear Counterstain : Hoechst 33342.

Supplier Data

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (AB227223)

Smad3 was immunoprecipitated from HeLa (human epitheilal cell line from cervix adenocarcinoma) whole cell lysate with 2 μg of ab227223. Western blot was performed from the immunoprecipitate using ab227223 at 1/1000 dilution. Anti-rabbit IgG was used as secondary antibody.

Lane 1 : HeLa whole cell lysate 50 μg (Input).

Lane 2 : Preimmune rabbit IgG instead of ab227223 in HeLa whole cell lysate.

Lane 3 : ab227223 IP in HeLa whole cell lysate.

All lanes:

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (ab227223)

Predicted band size: 48 kDa

false

ChIP - Anti-Smad3 antibody - ChIP Grade (AB227223)

ChIP

Supplier Data

ChIP - Anti-Smad3 antibody - ChIP Grade (AB227223)

Chromatin was prepared from Hela (human epithelial cell line from cervix adenocarcinoma) whole cell lysate. The ChIP was performed with 5 μg of preimmune rabbit IgG (Lane 1) and 5 μg of ab227223 (Lane 2). The immunoprecipitated DNA was detected by PCR with a primer set targeting to the smad7 promoter.

Supplier Data

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (AB227223)

Immunoprecipitation of SMAD2 / SMAD3 protein from HeLa whole cell extracts using 2 µg of SMAD2 / SMAD3 antibody.
Western blot analysis was performed using SMAD2 / SMAD3 antibody at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (ab227223) at 1/1000 dilution

Lane 1:

HeLa whole cell extracts (Input)

Lane 2:

HeLa whole cell extracts with control IgG (-)

Lane 3:

HeLa whole cell extracts (+)

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Observed band size: 60 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ChIP, ICC/IF, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human SMAD3. The exact immunogen used to generate this antibody is proprietary information.

P84022

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/1000", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.

Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

See full target information SMAD3

대체 명칭 보기

MADH3, SMAD3, SMAD family member 3, SMAD 3, hSMAD3, JV15-2, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록and refine your search

BMC nephrology 25:64 PubMed38395806

2024

Assessing the effects of tempol on renal fibrosis, inflammation, and oxidative stress in a high-salt diet combined with 5/6 nephrectomy rat model: utilizing oxidized albumin as a biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Beibei Liu,Yanling Hu,Danyang Tian,Jianlong Dong,Bing-Feng Li

Nature genetics 56:85-99 PubMed38092881

2023

A chromatin-regulated biphasic circuit coordinates IL-1β-mediated inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ezio T Fok,Simone J C F M Moorlag,Yutaka Negishi,Laszlo A Groh,Jéssica Cristina Dos Santos,Cathrin Gräwe,Valerie Villacorta Monge,Daphne D D Craenmehr,Mellanie van Roosmalen,David Pablo da Cunha Jolvino,Letícia Busato Migliorini,Ary Serpa Neto,Patricia Severino,Michiel Vermeulen,Leo A B Joosten,Mihai G Netea,Stephanie Fanucchi,Musa M Mhlanga

Oncogene 43:47-60 PubMed37935976

2023

Low expression of ZFP36L1 in osteosarcoma promotes lung metastasis by inhibiting the SDC4-TGF-β signaling feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjun Ma,Jiahao Zhuang,Hongyu Li,Rujia Mi,Yihui Song,Wen Yang,Yixuan Lu,Xin Shen,Yanfeng Wu,Huiyong Shen

BMC cardiovascular disorders 22:17 PubMed35081907

2022

Resveratrol ameliorates myocardial fibrosis by regulating Sirt1/Smad3 deacetylation pathway in rat model with dilated cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Qingquan Chen,Yu Zeng,Xiulin Yang,Yue Wu,Shuyu Zhang,Shirong Huang,Yameng Zhong,Min Chen

Frontiers in pharmacology 12:646240 PubMed34177571

2021

Resveratrol Ameliorates Cardiac Remodeling in a Murine Model of Heart Failure With Preserved Ejection Fraction.

Applications

Unspecified application

Species

Unspecified reactive species

Liyun Zhang,Juan Chen,Lianhua Yan,Qin He,Han Xie,Manhua Chen

Molecular therapy. Nucleic acids 22:673-683 PubMed33230465

2020

Overexpression of circRNA circUCK2 Attenuates Cell Apoptosis in Cerebral Ischemia-Reperfusion Injury via miR-125b-5p/GDF11 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wanghao Chen,Hong Wang,Jia Feng,Lukui Chen

BMC veterinary research 15:48 PubMed30709353

2019

All-trans retinoic acid inhibits lipopolysaccharide-induced inflammatory responses in bovine adipocytes via TGFβ1/Smad3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiushi Xu,Hongdou Jia,Li Ma,Guowen Liu,Chuang Xu,Ying Li,Xinwei Li,Xiaobing Li

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Smad3 antibody - ChIP Grade

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 antibody - ChIP Grade (AB227223)

Immunocytochemistry/ Immunofluorescence - Anti-Smad3 antibody - ChIP Grade (AB227223)

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (AB227223)

ChIP - Anti-Smad3 antibody - ChIP Grade (AB227223)

Immunoprecipitation - Anti-Smad3 antibody - ChIP Grade (AB227223)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (7)

Product promise