AB63402

Anti-Smad3 (phospho S204) antibody

Be the first to review this product! 리뷰 제출

(9 제품이 사용된 논문 )

Rabbit Polyclonal SMAD3 phospho S204 antibody. Suitable for WB and reacts with Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 pS204.

1 이미지

Unknown

Western blot - Anti-Smad3 (phospho S204) antibody (AB63402)

The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.

All lanes:

Western blot - Anti-Smad3 (phospho S204) antibody (ab63402) at 1/500 dilution

Lane 1:

Extracts from NIH/3T3 cells, treated with Serum (20%, 15mins)

Lane 2:

Extracts from NIH/3T3 cells, treated with Serum (20%, 15mins) plus phospho peptide

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human SMAD3 pS204. The exact immunogen used to generate this antibody is proprietary information.

P84022

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.

Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

See full target information SMAD3 pS204

대체 명칭 보기

MADH3, SMAD3, SMAD family member 3, SMAD 3, hSMAD3, JV15-2, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3

style

left-column

제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Allergologia et immunopathologia 52:91-96 PubMed38970271

2024

Jolkinolide B attenuates allergic airway inflammation and airway remodeling in asthmatic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Lin,Chao Xu,Jintong Ge,Hua Wu,Qi Wang

Biomolecules & biomedicine : PubMed38059910

2023

Mechanism of adipose tissue-derived stromal cell-extracellular vesicles in treating oral submucous fibrosis by blocking the TGF-β1/Smad3 pathway via the miR-760-3p/IGF1R axis

Applications

Unspecified application

Species

Unspecified reactive species

Fengcong Wang,Li Jiang,Ping Liu,Yanjun Jiang

Pharmacology 108:432-443 PubMed37343534

2023

Quercetin Alleviates Asthma-Induced Airway Inflammation and Remodeling through Downregulating Periostin via Blocking TGF-β1/Smad Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Fang,Wenwen Jin,Zhen Guo,Jumei Hao

Journal of cell science 134: PubMed34338295

2021

A dual role of YAP in driving TGFβ-mediated endothelial-to-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia Savorani,Matteo Malinverno,Roberta Seccia,Claudio Maderna,Monica Giannotta,Linda Terreran,Eleonora Mastrapasqua,Stefano Campaner,Elisabetta Dejana,Costanza Giampietro

Cancer biology & therapy 21:994-1004 PubMed33054513

2020

Cyclin E overexpression confers resistance to trastuzumab through noncanonical phosphorylation of SMAD3 in HER2+ breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph T Decker,Pridvi Kandagatla,Lei Wan,Regan Bernstein,Jeffrey A Ma,Lonnie D Shea,Jacqueline S Jeruss

Experimental lung research 45:245-254 PubMed31496319

2019

Atrial natriuretic peptide inhibits epithelial-mesenchymal transition (EMT) of bronchial epithelial cells through cGMP/PKG signaling by targeting Smad3 in a murine model of allergic asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyuan Chu,Xiufeng Zhang,Yabing Sun,Yaxi Liang,Jingyi Sun,Minyan Lu,Jianwei Huang,Ming Jiang,Libing Ma

Oncology reports 41:224-234 PubMed30542739

2018

sFRP1 exerts effects on gastric cancer cells through GSK3β/Rac1‑mediated restraint of TGFβ/Smad3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Xiang Peng,Shun-Yu Liang,Li Li

Cancer biology & therapy 19:271-281 PubMed29053396

2018

Embryonic stem cell secreted factors decrease invasiveness of triple-negative breast cancer cells through regulome modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Tarasewicz,Robert S Oakes,Misael O Aviles,Joelle Straehla,Kathryn M Chilton,Joseph T Decker,Jia Wu,Lonnie D Shea,Jacqueline S Jeruss

Experimental cell research 319:2928-37 PubMed24080014

2013

Serine-204 in the linker region of Smad3 mediates the collagen-I response to TGF-β in a cell phenotype-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

J A Browne,X Liu,H W Schnaper,T Hayashida

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Smad3 (phospho S204) antibody

Western blot - Anti-Smad3 (phospho S204) antibody (AB63402)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (9)

Product promise