AB193297

Anti-Smad3 (phospho T179) antibody

Be the first to review this product! 리뷰 제출

(13 제품이 사용된 논문 )

Rabbit Polyclonal SMAD3 phospho T179 antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 pT179 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

MADH3, SMAD3, SMAD family member 3, SMAD 3, hSMAD3, JV15-2, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3

1 이미지

Supplier Data

Western blot - Anti-Smad3 (phospho T179) antibody (AB193297)

All lanes:

Western blot - Anti-Smad3 (phospho T179) antibody (ab193297) at 1/500 dilution

Lane 1:

extracts from HeLa cells treated with TNF

Lane 2:

extracts from HeLa cells treated with TNF with antigen-specific peptide

Predicted band size: 48 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human SMAD3 pT179 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P84022

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab193297 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.

Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

See full target information SMAD3 pT179

style

left-column

제품이 사용된 논문 (13)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental and therapeutic medicine 22:1457 PubMed34737797

2021

Electroacupuncture regulates inflammation, collagen deposition and macrophage function in skeletal muscle through the TGF-β1/Smad3/p38/ERK1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Han,Ming Li,Huilin Liu,Haohan Li

Evidence-based complementary and alternative medicine : eCAM 2021:5649575 PubMed34422075

2021

Antifibrotic Effects of Kangxian Ruangan Capsule on Rats with Nonalcoholic Fatty Liver Fibrosis and Hepatic Stellate Cells through Regulation of TGF- and TLR4 Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Liu,Ying Zhou,Dan Dai,Hongmei Xia,Kang Zhao,Jianjun Zhang

Frontiers in molecular biosciences 8:697773 PubMed34222341

2021

LINC00174 Facilitates Cell Proliferation, Cell Migration and Tumor Growth of Osteosarcoma Regulating the TGF-β/SMAD Signaling Pathway and Upregulating SSH2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Changjun Zheng,Ronghang Li,Shuang Zheng,Hongjuan Fang,Meng Xu,Lei Zhong

Journal of orthopaedic translation 29:30-41 PubMed34094856

2021

Losartan protects against osteoarthritis by repressing the TGF-β1 signaling pathway upregulation of PPARγ.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhan Deng,Fei Chen,Yuwei Liu,Jinping Wang,Wei Lu,Wei Jiang,Weimin Zhu

British journal of pharmacology 178:1037-1054 PubMed33305374

2021

The antagonistic effects and mechanisms of microRNA-26a action in hypertensive vascular remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Zhang,Qiaozhu Wang,Xin Xing,Lijun Yang,Min Xu,Chunhui Cao,Rong Wang,Weicheng Li,Xiaolin Niu,Dengfeng Gao

Brazilian journal of medical and biological resear 54:e9206 PubMed33503202

2021

MicroRNA-302b mitigates renal fibrosis via inhibiting TGF-β/Smad pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Mengkui Sun,Wei Zhou,Fei Yao,Jianming Song,Yanan Xu,Zhimei Deng,Hongwang Diao,Shoulin Li

Drug design, development and therapy 14:5431-5439 PubMed33324040

2020

Xanthohumol Inhibits TGF-β1-Induced Cardiac Fibroblasts Activation via Mediating PTEN/Akt/mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanhao Jiang,Ning Xie,Taoli Sun,Wanjun Ma,Bikui Zhang,Wenqun Li

Journal of the American Heart Association 9:e017970 PubMed32865120

2020

MicroRNA-26a Protects the Heart Against Hypertension-Induced Myocardial Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Zhang,Qiaozhu Wang,Yanjing Feng,Xuegui Chen,Lijun Yang,Min Xu,Xiaofang Wang,Weicheng Li,Xiaolin Niu,Dengfeng Gao

Acta pharmacologica Sinica 41:1234-1245 PubMed32327724

2020

Nintedanib inhibits keloid fibroblast functions by blocking the phosphorylation of multiple kinases and enhancing receptor internalization.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Ya Zhou,Wen-Bo Wang,Xiao-Li Wu,Wen-Jie Zhang,Guang-Dong Zhou,Zhen Gao,Wei Liu

Bioscience reports 39: PubMed31729530

2019

Moracin attenuates LPS-induced inflammation in nucleus pulposus cells via Nrf2/HO-1 and NF-κB/TGF-β pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ronghe Gu,Zonggui Huang,Huijiang Liu,Qiwen Qing,Zou Zhuan,Lijing Yang,Zhongyi Su,Weiguo Huang

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Smad3 (phospho T179) antibody

Western blot - Anti-Smad3 (phospho T179) antibody (AB193297)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (13)

Product promise