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AB240605

Anti-SmarcAL1 antibody

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Rabbit Polyclonal SmarcAL1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SMARCAL1 aa 900 to C-terminus.

대체 명칭 보기

HARP, SMARCAL1, SNF2 related chromatin remodeling annealing helicase 1, HepA-related protein, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, Sucrose nonfermenting protein 2-like 1, hHARP

2 이미지
Immunoprecipitation - Anti-SmarcAL1 antibody (AB240605)
  • IP

Supplier Data

Immunoprecipitation - Anti-SmarcAL1 antibody (AB240605)

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. For western blotting, ab240605 was used at 0.1 μg/ml.

Lane 1 : ab240605 used for IP at 3 μg/mg lysate.
Lane 2 : Control IgG IP.

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-SmarcAL1 antibody (ab240605)

Predicted band size: 106 kDa

true

Exposure time: 3min

Western blot - Anti-SmarcAL1 antibody (AB240605)
  • WB

Supplier Data

Western blot - Anti-SmarcAL1 antibody (AB240605)

All lanes:

Western blot - Anti-SmarcAL1 antibody (ab240605) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 106 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human SMARCAL1 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NZC9

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대체품

Primary Antibodies

AB154226

Anti-SmarcAL1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SmarcAL1 antibody (AB154226)

  • 4
  • 2
Primary Antibodies

AB236786

Anti-SmarcAL1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SmarcAL1 antibody (AB236786)

  • 0
  • 0

보완 제품

Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

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Primary Antibodies

AB131272

Anti-ACTL6A antibody [EPR7443]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL6A antibody [EPR7443] (AB131272)

  • 0
  • 0
Primary Antibodies

AB32537

Anti-ERK1 antibody [Y72]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

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Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SmarcAL1 also known as SWI/SNF-related ATPase family member A1 is a DNA-dependent ATPase that unwinds DNA during replication and repair. It also plays a role in chromatin remodeling. The molecular mass of SmarcAL1 is approximately 105 kDa. SmarcAL1 expression is particularly noticeable in tissues with high replicative demands like testis and ovary among others.
Biological function summary

SmarcAL1 facilitates proper replication fork stability and functioning by associating with other proteins and complexes. It is a part of the SWI/SNF chromatin remodeling complex which alters the structure of chromatin to regulate access to DNA. This protein works with other chromatin remodeling factors to ensure the DNA replication and repair processes run smoothly.

Pathways

SmarcAL1 is a significant player in DNA damage response and repair pathways. It is actively involved in the homologous recombination repair pathway. Associated proteins within this pathway include BRCA1 and RAD51 which are essential for repairing double-strand breaks in DNA. Together these proteins promote genomic stability by correcting DNA damage and supporting normal cell cycle progression.

Mutations or dysfunctions in SmarcAL1 have connections to Schimke immuno-osseous dysplasia (SIOD) and various cancer types. These associations arise because SmarcAL1 influences genome stability and cell cycle progression. The connection of SmarcAL1 with BRCA1 highlights its relevance in breast cancer where malfunction in DNA repair mechanisms contribute to cancer development.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks.
See full target information SMARCAL1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell metabolism 32:420-436.e12 PubMed32768387

2020

Sirt2 Inhibition Enhances Metabolic Fitness and Effector Functions of Tumor-Reactive T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Imene Hamaidi,Lin Zhang,Nayoung Kim,Min-Hsuan Wang,Cristina Iclozan,Bin Fang,Min Liu,John M Koomen,Anders E Berglund,Sean J Yoder,Jiqiang Yao,Robert W Engelman,Ben C Creelan,Jose R Conejo-Garcia,Scott J Antonia,James J Mulé,Sungjune Kim
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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