AB235183

Anti-Smoothened antibody

(1 리뷰)

(7 제품이 사용된 논문 )

Rabbit Polyclonal Smoothened antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

대체 명칭 보기

SMOH, SMO, Protein smoothened, Protein Gx

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothened antibody (AB235183)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothened antibody (AB235183)

Paraffin-embedded human lung tissue stained for Smoothened using ab235183 at 1/100 dilution in immunohistochemical analysis. Counterstained with hematoxylin.

ab235183 developed a specific nuclear staining of the alveolar cells in lung tissue.

Supplier Data

Western blot - Anti-Smoothened antibody (AB235183)

Overexpressing lysate expected to run ~100 kDa.

All lanes:

Western blot - Anti-Smoothened antibody (ab235183) at 1/1000 dilution

Lane 1:

Smoothened (C-terminal DDK tagged) overexpressing HEK-293T lysate at 10 µg

Lane 2:

HEK293T lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/70000 dilution

Predicted band size: 86 kDa

false

Exposure time: 9s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Smoothened (SMO) is a protein that plays a vital role in the Hedgehog signaling pathway. It is also known by other names such as SMOH and Smoothened homolog. This protein has a molecular mass of approximately 86 kDa. Smoothened is expressed in a variety of tissues including the brain lungs and kidneys. It functions as a transmembrane protein with seven helices which transmits signals across the cell membrane.

Biological function summary

Smoothened influences cellular processes by participating in the Hedgehog signaling pathway. It operates as part of a complex with the Patched (PTCH) receptor. When the Hedgehog ligand binds to PTCH Smoothened becomes activated. This activation leads to changes in gene expression that control cell growth and differentiation. Smoothened is critical in embryonic development tissue regeneration and stem cell maintenance.

Pathways

Smoothened interacts with essential components of the Hedgehog signaling pathway. This pathway includes proteins like PTCH and Gli transcription factors. Smoothened activation triggers the release of these Gli proteins from inhibitory complexes which then migrate to the nucleus to modulate the transcription of target genes. The Hedgehog pathway is important in regulating developmental processes and cellular responses.

Smoothened has connections to various forms of cancer including basal cell carcinoma and medulloblastoma. Abnormal activation or mutations in Smoothened can lead to unchecked cell proliferation. Smoothened also shows relevance in the development of gliomas. Drugs like 'anti smo' or 'glasdegib' act as Smoothened antagonists aiming to inhibit its function in these diseases by targeting the Hedgehog signaling pathway.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed : 19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).

See full target information SMO

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Balkan medical journal 42:440-451 PubMed40888349

2025

Inhibition of PI3K and Hedgehog Signaling Pathway Inhibits Hypoxia-Induced Vasculogenic Mimicry Formation in Ovarian Cancer Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liang,Yun Bai,Huan Zhao

Molecular biology reports 50:3317-3327 PubMed36725745

2023

Sirt1 regulates microglial activation and inflammation following oxygen-glucose deprivation/reoxygenation injury by targeting the Shh/Gli-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Liao,Jiagui Huang,Jie Liu,Huimin Zhu,Yue Chen,Xuemei Li,Jun Wen,Qin Yang

Journal of ovarian research 15:141 PubMed36581994

2022

GIMAP7 induces oxidative stress and apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inhibiting sonic hedgehog signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Anran Xu,Yuanyuan Fan,Song Liu,Lianbing Sheng,Yanyan Sun,Huijun Yang

BioMed research international 2022:8453011 PubMed36246980

2022

Resveratrol Induces Apoptosis, Suppresses Migration, and Invasion of Cervical Cancer Cells by Inhibiting the Hedgehog Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Jiang,Zhenghua Liu,Xiuping Zhou,Fenglan Peng,Zhihui Wang,Feng Li,Ming Li

Experimental and therapeutic medicine 23:429 PubMed35607374

2022

Expression of yes-associated protein, β-catenin and smoothened, and their clinical significance in invasive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pengju Liu,Jianfeng Zeng,Gaohua Yang

Frontiers in cell and developmental biology 9:716610 PubMed34646822

2021

Sonic Hedgehog Induces Mesenchymal Stromal Cell Senescence-Associated Secretory Phenotype and Chondrocyte Apoptosis in Human Osteoarthritic Cartilage.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Feng,Wenguang Liu,Jing Ding,Yusheng Qiu,Qian Chen

Molecular medicine reports 23: PubMed33398379

2021

Silencing of long non‑coding RNA NEAT1 inhibits hepatocellular carcinoma progression by downregulating SMO by sponging microRNA‑503.

Applications

Unspecified application

Species

Unspecified reactive species

Chuihua Sun,Ting Xiao,Ying Xiao,Yunbao Li

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Smoothened antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothened antibody (AB235183)

Western blot - Anti-Smoothened antibody (AB235183)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (7)

Product promise