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AB15716

Anti-SMUG1 antibody

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(2 제품이 사용된 논문 )

Goat Polyclonal SMUG1 antibody. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Single-strand selective monofunctional uracil DNA glycosylase aa 1-50.

대체 명칭 보기

Single-strand selective monofunctional uracil DNA glycosylase, SMUG1

3 이미지
Immunocytochemistry - Anti-SMUG1 antibody (AB15716)
  • ICC

Supplier Data

Immunocytochemistry - Anti-SMUG1 antibody (AB15716)

Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling SMUG1 with ab15716 at 10 μg/mL showing strong nuclear and cytoplasmic staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 μg/mL (green). Nuclear DNA was labelled with DAPI (blue).

Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL).

Immunocytochemistry - Anti-SMUG1 antibody (AB15716)
  • ICC

Supplier Data

Immunocytochemistry - Anti-SMUG1 antibody (AB15716)

Immunocytochemistry/immunofluorescence analysis of U2OS cells labelling SMUG1 with ab15716 at 10 μg/mL showing strong nuclear staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 μg/mL (green). Nuclear DNA was labelled with DAPI (blue).

Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL).

Flow Cytometry (Intracellular) - Anti-SMUG1 antibody (AB15716)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SMUG1 antibody (AB15716)

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) labelling SMUG1 with ab15716. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (1 μg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human Single-strand selective monofunctional uracil DNA glycosylase aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q53HV7

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대체품

Primary Antibodies

AB192240

Anti-SMUG1 antibody [EPR15624]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (AB192240)

  • 5
  • 3

보완 제품

Primary Antibodies

AB76420

Anti-RPA32/RPA2 antibody [EPR2877Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)

  • 5
  • 1
Primary Antibodies

AB110638

Anti-PMS2 antibody [EPR3947]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] (AB110638)

  • 4
  • 3
Primary Antibodies

AB79398

Anti-RPA70 antibody [EPR3472]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-RPA70 antibody [EPR3472] (AB79398)

  • 5
  • 6
Primary Antibodies

AB92312

Anti-MLH1 antibody [EPR3894]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (AB92312)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
보관 버퍼
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SMUG1 also known as Single-strand-selective Monofunctional Uracil-DNA Glycosylase 1 plays a role in DNA repair mechanisms. It is an enzyme involved in the base excision repair (BER) pathway specializing in the removal of uracil from single-stranded DNA. SMUG1 has a molecular weight of about 33 kDa. The protein is expressed in various tissues with notable presence in the brain liver and lung. Its expression levels adjust according to the tissue's need for active DNA repair.
Biological function summary

The process of DNA metabolism relies heavily on mechanisms related to the SMUG1 enzyme. As part of the DNA repair machinery it identifies and excises uracil bases from DNA which result from deamination of cytosine or misincorporation during DNA synthesis. SMUG1 does not function within a larger protein complex but interacts dynamically with other repair proteins like AP endonucleases. This activity helps maintain genomic stability by preventing mutations that could arise from uracil incorporation.

Pathways

SMUG1 contributes importantly to the base excision repair pathway an important process for DNA maintenance. This pathway addresses small non-helix-distorting base lesions such as deaminated oxidized or alkylated bases. Within this pathway SMUG1's activity aligns with other glycosylases and the APEX1 protein which processes the abasic sites left after SMUG1 removes uracil. Furthermore SMUG1's function supports the overall cellular DNA damage response signaling when repair and further processing are necessary.

Alterations in the SMUG1 protein have connections to cancer and neurodegeneration. Loss of SMUG1 activity may result in accumulation of mutations due to unprocessed uracil in the DNA contributing to tumorigenesis. In particular studies have shown links between SMUG1 and glioma where defective DNA repair mechanisms escalate mutation rates. Also in neurodegenerative disorders it contributes directly through genomic instability with connections to APEX1 showing potential overlapping repair functions critical for neuron maintenance.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following : ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.
See full target information Single-strand selective monofunctional uracil DNA glycosylase
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nucleic acids research 42:1698-710 PubMed24178031

2013

Vpr expression abolishes the capacity of HIV-1 infected cells to repair uracilated DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Eldin,Nathalie Chazal,David Fenard,Eric Bernard,Jean-François Guichou,Laurence Briant

FASEB journal : official publication of the Federa 25:4406-14 PubMed21891782

2011

p73 protein regulates DNA damage repair.

Applications

WB

Species

Human

Elena Zaika,Jinxiong Wei,Dengping Yin,Claudia Andl,Ute Moll,Wael El-Rifai,Alexander I Zaika
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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