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AB236081

Anti-SMURF1 antibody

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(7 제품이 사용된 논문 )

Rabbit Polyclonal SMURF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMURF1 aa 150 to C-terminus.

대체 명칭 보기

KIAA1625, SMURF1, E3 ubiquitin-protein ligase SMURF1, hSMURF1, HECT-type E3 ubiquitin transferase SMURF1, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)

Immunofluorescent analysis of 4% formaldehyde fixed, 0.2% Triton X-100 permeabolized A549 (Human lung carcinoma cell line) cells labeling SMURF1 with ab236081 at 1/50 dilution, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody (green). Counterstained with DAPI.

Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for SMURF1 using ab236081 at 1/100 dilution in ICC/IF. An Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for SMURF1 using ab236081 at 1/100 dilution in ICC/IF. An Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SMURF1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9HCE7

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대체품

Primary Antibodies

AB57573

Anti-SMURF1 antibody [1D7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMURF1 antibody [1D7] (AB57573)

  • 0
  • 0

보완 제품

Primary Antibodies

AB140601

Anti-Ubiquitin (linkage-specific K48) antibody [EP8589]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Ubiquitin (linkage-specific K48) antibody [EP8589] (AB140601)

  • 5
  • 10
Primary Antibodies

AB76245

Anti-Galectin 3 antibody [EP2775Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [EP2775Y] (AB76245)

  • 5
  • 6
Primary Antibodies

AB313470

Anti-SMURF2 antibody [RP23040176]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SMURF2 Antibody [RP23040176] (AB313470)

  • 0
  • 0
Primary Antibodies

AB18184

Anti-6X His tag® antibody [HIS.H8]

Lab Essentials

Western blot - Anti-6X His tag® antibody [HIS.H8] (AB18184)

  • 5
  • 32
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SMURF1 also known as SMAD-specific E3 ubiquitin protein ligase 1 functions as an E3 ubiquitin ligase involved in the targeted degradation of proteins. This protein weighs approximately 72 kDa and expresses primarily in various human tissues including bone lung and kidney. SMURF1 plays an essential role in regulating protein turnover by tagging substrates for proteasomal degradation. It interacts with substrates through a C2 domain which aids in membrane localization and substrate recognition effectively controlling cellular protein levels and their functions.
Biological function summary

SMURF1 influences numerous cellular processes such as bone morphogenetic protein (BMP) signaling by regulating the availability and stability of signaling molecules. It does not function in isolation; it is a component of a ubiquitin-proteasome system complex that includes other cofactors assisting its activity. SMURF1 therefore affects cell growth differentiation and homeostasis by managing signal transduction pathways critical for these cellular activities.

Pathways

SMURF1 is involved in the BMP and Wnt signaling pathways. Both are fundamental for various biological activities including embryonic development and cell cycle regulation. Within these pathways SMURF1 influences the activity of SMAD6 and related proteins modulating their turnover and impact on downstream signaling processes. These pathways also interact with other cellular pathways but SMURF1's specific role remains concentrated on modulating protein ubiquitination events.

SMURF1 has been linked to cancer and osteoporosis. Its dysregulation can disrupt normal cellular signaling contributing to tumorigenesis or impaired bone maintenance. In cancer its interaction with proteins such as SMAD7 can alter cell signaling promoting uncontrolled cell growth. In osteoporosis SMURF1's regulation of bone remodeling processes might be compromised leading to decreased bone mass and fragility. Understanding SMURF1's roles may open avenues for therapeutic interventions in these disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed : 23087404). Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed : 21791611). Plays a role in dendrite formation by melanocytes (PubMed : 23999003).
See full target information SMURF1
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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Autophagy 21:530-547 PubMed39324484

2024

SMURF1 mediates damaged lysosomal homeostasis by ubiquitinating PPP3CB to promote the activation of TFEB.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Xia,Xuan Liu,Lu Zhong,Jun Qu,Lei Dong

Autophagy 20:735-751 PubMed37909662

2023

SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Xia,Hanfei Zheng,Yang Li,Wanting Xu,Chengwei Wu,Jiachen Xu,Shanhu Li,Lingqiang Zhang,Lei Dong

Laboratory investigation; a journal of technical methods and pathology 102:1121-1131 PubMed35710596

2022

PAQR3 depletion accelerates diabetic wound healing by promoting angiogenesis through inhibiting STUB1-mediated PPARγ degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Qiu,Chang Shu,Xin Li,Wei-Chang Zhang

Bioengineered 13:2371-2386 PubMed35034538

2022

miR-129-5p restores cardiac function in rats with chronic heart failure by targeting the E3 ubiquitin ligase Smurf1 and promoting PTEN expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Qi,Yan Tang,Lu Yin,Keke Ding,Cuimei Zhao,Wenwen Yan,Yi'an Yao

Aging 13:12239-12257 PubMed33872218

2021

Extracellular-vesicle containing miRNA-503-5p released by macrophages contributes to atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuquan Wang,Zhengmin Xu,Xiaoli Wang,Jiankang Zheng,Lihan Peng,Yunfei Zhou,Yongyan Song,Zhan Lu

British journal of pharmacology 178:2246-2265 PubMed33085791

2021

Regulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signalling via interaction with Gli1.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhang,Feixia Wang,Jianlin He,Naqi Lian,Zhenyi Wang,Jiangjuan Shao,Hai Ding,Shanzhong Tan,Anping Chen,Zili Zhang,Shijun Wang,Shizhong Zheng

Frontiers in medicine 7:577578 PubMed33425934

2020

Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-25 Regulates the Ubiquitination and Degradation of Runx2 by SMURF1 to Promote Fracture Healing in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yikun Jiang,Jun Zhang,Zhengwei Li,Guoliang Jia
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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