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AB5666

Anti-SNAP25 antibody

5

(5 리뷰들)

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(62 제품이 사용된 논문 )

Rabbit Polyclonal SNAP25 antibody. Suitable for ICC, Flow Cyt, WB and reacts with Rat, Mouse samples. Cited in 62 publications. Immunogen corresponding to Synthetic Peptide within Mouse Snap25 aa 150-250.
4 이미지
Immunocytochemistry - Anti-SNAP25 antibody (AB5666)
  • ICC

Unknown

Immunocytochemistry - Anti-SNAP25 antibody (AB5666)

ICC/IF image of ab5666 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry - Anti-SNAP25 antibody (AB5666)
  • ICC

Supplier Data

Immunocytochemistry - Anti-SNAP25 antibody (AB5666)

Immunlocalization of SNAP-25 in cultured rat hippocampal cells using ab5666.

Western blot - Anti-SNAP25 antibody (AB5666)
  • WB

Supplier Data

Western blot - Anti-SNAP25 antibody (AB5666)

All lanes:

Western blot - Anti-SNAP25 antibody (ab5666)

Lane 1:

Mouse cerebellum at 30 µg

Lane 2:

Mouse brain at 30 µg

Lane 3:

Rat brain at 30 µg

Lane 4:

Membrane enriched extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate

Predicted band size: 23 kDa

false

Western blot - Anti-SNAP25 antibody (AB5666)
  • WB

AbReview11837****

Western blot - Anti-SNAP25 antibody (AB5666)

All lanes:

Western blot - Anti-SNAP25 antibody (ab5666) at 1/10000 dilution

All lanes:

Whole tissue lysate prepared from mouse hippocampus

Secondary

All lanes:

HRP conjugated goat anti-rabbit Ig at 1/10000 dilution

Predicted band size: 23 kDa

Observed band size: 25 kDa

true

Exposure time: 20s

This image is courtesy of an anonymous Abreview

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

ICC, Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Mouse Snap25 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

P60879

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대체품

Primary Antibodies

AB109105

Anti-SNAP25 antibody [EPR3275]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] (AB109105)

  • 5
  • 3
Primary Antibodies

AB41455

Anti-SNAP25 antibody

Western blot - Anti-SNAP25 antibody (AB41455)

  • 5
  • 2
Primary Antibodies

AB207366

Alexa Fluor® 647 Anti-SNAP25 antibody [EP3274]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SNAP25 antibody [EP3274] (AB207366)

  • 0
  • 0
Primary Antibodies

AB208379

Alexa Fluor® 488 Anti-SNAP25 antibody [EP3274]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SNAP25 antibody [EP3274] (AB208379)

  • 0
  • 0
Primary Antibodies

AB108990

Anti-SNAP25 antibody [EP3274]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (AB108990)

  • 5
  • 2
Primary Antibodies

AB226046

Anti-SNAP25 antibody [EP3274] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (AB226046)

  • 4
  • 1
Primary Antibodies

AB171355

Anti-SNAP25 antibody [EPR3275] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] - BSA and Azide free (AB171355)

  • 0
  • 0

보완 제품

Primary Antibodies

AB32127

Anti-Synaptophysin antibody [YE269] - Synaptic Marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB32127)

  • 5
  • 39
Primary Antibodies

AB192757

Anti-PSD95 antibody [K28/43] - Synaptic Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PSD95 antibody [K28/43] - Synaptic Marker (AB192757)

  • 0
  • 0
Primary Antibodies

AB52623

Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (AB52623)

  • 5
  • 6
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 76
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Reactivity 정보

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.
Biological function summary

SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.

Pathways

One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.

SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

t-SNARE involved in the molecular regulation of neurotransmitter release (PubMed : 8103915, PubMed : 8243676). May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF (PubMed : 16672379). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells (By similarity).
See full target information Snap25

대체 명칭 보기

Snap, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP
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제품이 사용된 논문 (62)

Recent publications for all applications. Explore the 전체 목록 and refine your search

iScience 27:111156 PubMed39507243

2024

SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Vineeth Andisseryparambil Raveendran,Melissa Serranilla,Azam Asgarihafshejani,Miranda de Saint-Rome,Mariia Cherednychenko,Shanelle Mullany,Jennifer A Mitchell,Jessica C Pressey,Melanie A Woodin

EMBO molecular medicine 16:2795-2826 PubMed39402139

2024

Unraveling autophagic imbalances and therapeutic insights in Mecp2-deficient models.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Esposito,Tommaso Seri,Martina Breccia,Marzia Indrigo,Giuseppina De Rocco,Francesca Nuzzolillo,Vanna Denti,Francesca Pappacena,Gaia Tartaglione,Simone Serrao,Giuseppe Paglia,Luca Murru,Stefano de Pretis,Jean-Michel Cioni,Nicoletta Landsberger,Fabrizia Claudia Guarnieri,Michela Palmieri

NPJ Parkinson's disease 10:107 PubMed38773105

2024

A stem cell-based assay platform demonstrates alpha-synuclein dependent synaptic dysfunction in patient-derived cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew J White,Karis A Clark,Kellianne D Alexander,Nagendran Ramalingam,Tracy L Young-Pearse,Ulf Dettmer,Dennis J Selkoe,Gary P H Ho

Biology 12: PubMed37372046

2023

Brain-Derived Estrogen Regulates Neurogenesis, Learning and Memory with Aging in Female Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Huang,Wuxiang Sun,Fujia Gao,Haoran Ma,Tao Yuan,Zixuan Liu,Huiyu Liu,Jiewei Hu,Jing Bai,Xin Zhang,Ruimin Wang

Science signaling 16:eadd7220 PubMed36787382

2023

Palmitoylation of the Parkinson's disease-associated protein synaptotagmin-11 links its turnover to α-synuclein homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Gary P H Ho,Erin C Wilkie,Andrew J White,Dennis J Selkoe

The Journal of pharmacology and experimental therapeutics 383:117-128 PubMed36116796

2022

Metabotropic Glutamate Receptors Modulate Exocytotic Tau Release and Propagation.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Mazzo,Ioana Butnaru,Olivera Grubisha,Elena Ficulle,Helen Sanger,Griffin Fitzgerald,Feng Pan,Francesca Pasqui,Tracey Murray,James Monn,Xia Li,Michael Hutton,Suchira Bose,Giampietro Schiavo,Emanuele Sher

ASN neuro 14:17590914221121257 PubMed36017573

2022

Exposure to Zika Virus Results in sex-Specific Memory Deficits and Neurological Alterations in Adult Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Thiago A Andrade,Julia S Fahel,Jessica M de Souza,Ana C Terra,Danielle G Souza,Vivian V Costa,Mauro M Teixeira,Enrrico Bloise,Fabiola M Ribeiro

Frontiers in neuroscience 16:905403 PubMed35860293

2022

Ceftriaxone Suppresses Group II Metabotropic Glutamate Receptor Expression Contributing to Reversal of Recognition Memory Deficits of Amyloid Precursor Protein/Presenilin 1 AD Mice.

Applications

Unspecified application

Species

Unspecified reactive species

ShuJuan Fan,Li Li,LiRong Liu,He Li,XiaoHui Xian,WenBin Li

Brain pathology (Zurich, Switzerland) 32:e13056 PubMed35178783

2022

Glial activation in prion diseases is selectively triggered by neuronal PrP.

Applications

Unspecified application

Species

Unspecified reactive species

Asvin K K Lakkaraju,Silvia Sorce,Assunta Senatore,Mario Nuvolone,Jingjing Guo,Petra Schwarz,Rita Moos,Pawel Pelczar,Adriano Aguzzi

Frontiers in pharmacology 13:807330 PubMed35185566

2022

Caffeine Functions by Inhibiting Dorsal and Ventral Hippocampal Adenosine 2A Receptors to Modulate Memory and Anxiety, Respectively.

Applications

Unspecified application

Species

Unspecified reactive species

Yawei Xu,Yalei Ning,Yan Zhao,Yan Peng,Fen Luo,Yuanguo Zhou,Ping Li
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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