AB308182

Anti-SNAP91 antibody [HL1657]

Recombinant
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Rabbit Recombinant Monoclonal Snap91 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse, Cat samples.

5 이미지

Immunohistochemistry paraffin embedded sections - Anti-SNAP91 antibody [HL1657] (AB308182)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SNAP91 antibody [HL1657] (AB308182)

Immunohistochemical analysis of paraffin-embedded human glioblastoma labeling SNAP91 protein with ab308182 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Supplier Data

Western blot - Anti-SNAP91 antibody [HL1657] (AB308182)

Separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-SNAP91 antibody [HL1657] (ab308182) at 1/1000 dilution

All lanes:

Human brain tissue lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 91.9 kDa

false

Supplier Data

Western blot - Anti-SNAP91 antibody [HL1657] (AB308182)

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SNAP91 antibody [HL1657] (ab308182) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SNAP91 antibody [HL1657] (ab308182) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 50 µg

Lane 2:

Rat brain tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 91.9 kDa

false

Supplier Data

Western blot - Anti-SNAP91 antibody [HL1657] (AB308182)

All lanes:

Western blot - Anti-SNAP91 antibody [HL1657] (ab308182) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 91.9 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP91 antibody [HL1657] (AB308182)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP91 antibody [HL1657] (AB308182)

Immunohistochemical analysis of paraffin-embedded cat brain tissue labelling SNAP91 with ab308182 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1657

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse, Cat

Applications

WB, IHC-P

applications

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

AP180 also known as AP3K is a protein with a mass of about 90 kDa that has essential roles in endocytosis and synaptic vesicle recycling. This protein is expressed in the nervous system particularly in the brain. AP180 regulates clathrin-coated vesicle formation by facilitating the assembly of clathrin coats on vesicles. It interacts with clathrin through a specific domain ensuring that vesicles have the necessary structural components to capture and transport neurotransmitters effectively.

Biological function summary

AP180 ensures proper functioning of synaptic transmission by assisting in the regulation of vesicle trafficking. It forms part of a complex required for vesicle budding from the plasma membrane. AP180's ability to bind clathrin with high specificity and affinity makes it indispensable for maintaining the vesicular system's integrity. The protein participates in assembling clathrin lattices which are fundamental for creating adequately-sized synaptic vesicles critical for efficient neurotransmission.

Pathways

AP180 integrates into the synaptic vesicle cycle and endocytic pathway thereby influencing neural communication. In the context of the synaptic vesicle cycle AP180 works closely with proteins like synaptotagmin and synaptophysin for efficient neurotransmitter release and recycling. It also interacts within the endocytic pathway to regulate membrane retrieval an action important in synapses where neurotransmitter availability depends on this fast recycling process. These interactions highlight AP180's central role in maintaining synaptic function and stability.

AP180 connects to cognitive disorders and neurodegenerative diseases. Its malfunction might contribute to cognitive impairments as observed in Alzheimer's disease models due to its pivotal role in synaptic vesicle recycling and transmission. Moreover abnormalities in AP180 could influence Parkinson's disease pathways given its interaction with other synaptic proteins such as alpha-synuclein which is implicated in the disease. Therefore AP180 represents a significant target for understanding synaptic dysregulation in these neurodegenerative conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Snap91

대체 명칭 보기

91 kDa synaptosomal associated protein, AP 180, Assembly Protein 180 kDa, Assembly Protein AP180, Clathrin coat assembly protein, Clathrin coat assembly protein AP180, Clathrin coat associated protein AP180, DKFZp781O051, F120, KIAA0656, NP185, SNAP 91, Synaptosomal associated protein 91 kD homolog, Synaptosomal associated protein 91 kDa homolog, pp155

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SNAP91 antibody [HL1657]

Immunohistochemistry paraffin embedded sections - Anti-SNAP91 antibody [HL1657] (AB308182)

Western blot - Anti-SNAP91 antibody [HL1657] (AB308182)

Western blot - Anti-SNAP91 antibody [HL1657] (AB308182)

Western blot - Anti-SNAP91 antibody [HL1657] (AB308182)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP91 antibody [HL1657] (AB308182)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

보완 제품

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise