AB233644

Anti-SNF2L antibody [2H7B9]

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Mouse Monoclonal SNF2L antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCA1 aa 900 to C-terminus.

대체 명칭 보기

SNF2L, SNF2L1, SMARCA1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A1, Global transcription activator SNF2L1, Nucleosome-remodeling factor subunit SNF2L, SNF2 related chromatin remodeling ATPase 1

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody [2H7B9] (AB233644)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody [2H7B9] (AB233644)

Paraffin-embedded human stomach cancer tissue stained for SNF2L using ab233644 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody [2H7B9] (AB233644)

Paraffin-embedded human esophageal cancer tissue stained for SNF2L using ab233644 at 1/200 dilution in immunohistochemical analysis.

Flow Cytometry - Anti-SNF2L antibody [2H7B9] (AB233644)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-SNF2L antibody [2H7B9] (AB233644)

Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cell line labeling SNF2L with ab233644 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-SNF2L antibody [2H7B9] (AB233644)

Expected MW is 42.4 kDa.

All lanes:

Western blot - Anti-SNF2L antibody [2H7B9] (ab233644) at 1/500 dilution

All lanes:

Human SNF2L (aa 933-1070) recombinant protein

Predicted band size: 123 kDa

false

Supplier Data

Western blot - Anti-SNF2L antibody [2H7B9] (AB233644)

All lanes:

Western blot - Anti-SNF2L antibody [2H7B9] (ab233644) at 1/500 dilution

Lane 1:

PANC-1 (human pancreatic epithelial cancinoma cell line) cell lysate

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 3:

SW620 cell lysate

Lane 4:

Ht-29 (human colorectal adenocarcinoma cell line) cell lysate

Lane 5:

SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

Lane 6:

SK-OV-3 (human ovarian cancer cell line) cell lysate

Predicted band size: 123 kDa

false

Supplier Data

Western blot - Anti-SNF2L antibody [2H7B9] (AB233644)

All lanes:

Western blot - Anti-SNF2L antibody [2H7B9] (ab233644) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

SNF2L (aa 933-1070)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 123 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

2H7B9

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SMARCA1 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P28370

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SNF2L also known as SMARCA1 is a protein that functions as a chromatin remodeler. The molecular weight of SNF2L approximately 136 kDa. SNF2L is expressed widely with a higher presence in the brain and testis. The protein plays an active role in the regulation of gene expression by altering chromatin structure through ATP-dependent mechanisms. SNF2L belongs to the ISWI family of chromatin remodelers which facilitates the process by sliding or restructuring nucleosomes on DNA.

Biological function summary

SNF2L acts as part of the NURF (Nucleosome Remodeling Factor) complex. This complex is involved in modifying chromatin to make DNA accessible for transcription machinery. In addition to its role in transcription regulation SNF2L contributes to DNA replication and repair processes. By affecting chromatin architecture SNF2L influences the cell cycle and various developmental pathways. SNF2L's action is essential for maintaining proper gene expression patterns necessary for normal cellular function.

Pathways

SNF2L contributes to the Wnt signaling and neuronal differentiation pathways. In these contexts SNF2L works with proteins such as β-catenin in Wnt signaling where its chromatin remodeling abilities facilitate the transcription of Wnt target genes. Additionally SNF2L interacts with neural transcription factors during neuronal differentiation guiding the chromatin remodeling efforts necessary for neurogenesis. These pathways illustrate SNF2L's importance in regulating genes particularly those involved in cell fate decisions.

SNF2L has been associated with neurodevelopmental disorders and cancer. Mutations or dysregulation of SNF2L can lead to intellectual disabilities and other brain-related anomalies highlighting its significance in cognitive function. In cancer anomalies in the expression or mutation of SNF2L can influence tumor progression potentially through pathways involving other tumor-related chromatin remodelers like BRCA1. As a result understanding SNF2L can offer insights into therapeutic strategies for these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Isoform 1. ATPase that possesses intrinsic ATP-dependent chromatin-remodeling activity (PubMed : 14609955, PubMed : 15310751, PubMed : 15640247, PubMed : 28801535). ATPase activity is substrate-dependent, and is increased when nucleosomes are the substrate, but is also catalytically active when DNA alone is the substrate (PubMed : 14609955, PubMed : 15310751, PubMed : 15640247). Catalytic subunit of ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed : 14609955, PubMed : 15310751, PubMed : 15640247, PubMed : 28801535). Within the ISWI chromatin-remodeling complexes, slides edge- and center-positioned histone octamers away from their original location on the DNA template (PubMed : 28801535). Catalytic activity and histone octamer sliding propensity is regulated and determined by components of the ISWI chromatin-remodeling complexes (PubMed : 28801535). The BAZ1A-, BAZ1B-, BAZ2A- and BAZ2B-containing ISWI chromatin-remodeling complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template (PubMed : 28801535). The CECR2- and RSF1-containing ISWI chromatin-remodeling complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed : 28801535). Within the NURF-1 and CERF-1 ISWI chromatin remodeling complexes, nucleosomes are the preferred substrate for its ATPase activity (PubMed : 14609955, PubMed : 15640247). Within the NURF-1 ISWI chromatin-remodeling complex, binds to the promoters of En1 and En2 to positively regulate their expression and promote brain development (PubMed : 14609955). May promote neurite outgrowth (PubMed : 14609955). May be involved in the development of luteal cells (PubMed : 16740656). Facilitates nucleosome assembly during DNA replication, ensuring replication fork progression and genomic stability by preventing replication stress and nascent DNA gaps (PubMed : 39413208).. Isoform 2. Catalytically inactive when either DNA or nucleosomes are the substrate and does not possess chromatin-remodeling activity (PubMed : 15310751, PubMed : 28801535). Acts as a negative regulator of chromatin remodelers by generating inactive complexes (PubMed : 15310751).

See full target information SMARCA1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer immunology, immunotherapy : CII 71:1075-1091 PubMed34562123

2021

LncRNA MIR155HG induces M2 macrophage polarization and drug resistance of colorectal cancer cells by regulating ANXA2.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhou,Jian Li,Mingmei Liao,Qi Zhang,Mei Yang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SNF2L antibody [2H7B9]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody [2H7B9] (AB233644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody [2H7B9] (AB233644)

Flow Cytometry - Anti-SNF2L antibody [2H7B9] (AB233644)

Western blot - Anti-SNF2L antibody [2H7B9] (AB233644)

Western blot - Anti-SNF2L antibody [2H7B9] (AB233644)

Western blot - Anti-SNF2L antibody [2H7B9] (AB233644)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise