Anti-SNX4 antibody [EPR16954] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SNX4 antibody [EPR16954] - BSA and Azide free (AB236147)

Intracellular Flow Cytometry analysis of HeLa cells labelling SNX4 (red) with purified ab198504 at dilution of 1/60. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198504).

• IP

Supplier Data

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - BSA and Azide free (AB236147)

Western blot analysis of immunoprecipitation pellet from 1 mg 293 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

Lane 1 (Input) : 293 whole cell lysate 10µg.

Lane 2 (+) : 293 whole cell lysate using ab198504 at 1/1000 dilution.

Lane 3 (-) : 293 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198504).

All lanes:

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (<a href='/ko/products/primary-antibodies/snx4-antibody-epr16954-c-terminal-ab198504'>ab198504</a>)

Predicted band size: 52 kDa

true

Exposure time: 2min

• IP

Supplier Data

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - BSA and Azide free (AB236147)

Western blot analysis of immunoprecipitation pellet from 1 mg PC12 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

Lane 1 (Input) : PC12 whole cell lysate 10µg.

Lane 2 (+) : PC12 whole cell lysate using ab198504 at 1/1000 dilution.

Lane 3 (-) : PC12 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198504).

All lanes:

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (<a href='/ko/products/primary-antibodies/snx4-antibody-epr16954-c-terminal-ab198504'>ab198504</a>)

Predicted band size: 52 kDa

false