Anti-SNX4 antibody [EPR16954] - C-terminal

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Intracellular Flow Cytometry analysis of HeLa cells labelling SNX4 (red) with purified ab198504 at dilution of 1/60. The secondary antibody used was Alexa Fluorr^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

• IP

Supplier Data

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Western blot analysis of immunoprecipitation pellet from 1 mg 293 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

Lane 1 (Input) : 293 whole cell lysate 10μg.

Lane 2 (+) : 293 whole cell lysate using ab198504 at 1/1000 dilution.

Lane 3 (-) : 293 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

Predicted band size: 52 kDa

true

Exposure time: 2min

• IP

Supplier Data

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Western blot analysis of immunoprecipitation pellet from 1 mg PC12 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

Lane 1 (Input) : PC12 whole cell lysate 10μg.

Lane 2 (+) : PC12 whole cell lysate using ab198504 at 1/1000 dilution.

Lane 3 (-) : PC12 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

Predicted band size: 52 kDa

false

• WB

Supplier Data

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/5000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

true

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/10000 dilution

All lanes:

Mouse brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

true

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/1000 dilution

Lane 1:

C6 whole cell lysate at 10 µg

Lane 2:

RAW 264.7 whole cell lysate at 10 µg

Lane 3:

PC12 whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

true

Exposure time: 15s