AB67313

Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody

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(9 제품이 사용된 논문 )

Rabbit Polyclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody. Suitable for WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SLC9A1.

1 이미지

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Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67313)

Lane 1:

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67313) at 1 µg/mL

Lane 2:

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67313) at 2 µg/mL

All lanes:

Rat kidney tissue lysate at 15 µg

Predicted band size: 91 kDa

Observed band size: 50 kDa,70 kDa,91 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

IHC-FoFr, WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SLC9A1. The exact immunogen used to generate this antibody is proprietary information.

P19634

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium/Hydrogen Exchanger 1 (NHE-1) also known as NHE transporter or antisodium exchanger plays a significant role in regulating intracellular pH and cell volume by exchanging sodium ions for hydrogen ions across the plasma membrane. NHE-1 is a type of NHE protein with a molecular weight of approximately 85-110 kDa. This transmembrane protein is widely expressed in various tissues including the heart brain kidneys and skeletal muscles where it helps maintain cellular homeostasis.

Biological function summary

NHE-1 impacts cellular processes by modulating pH levels within cells and participating in the regulation of cell proliferation differentiation and apoptosis. It doesn’t function as part of a larger complex but plays an independent role essential to cellular ionic balance. The proper functioning of NHE-1 is critical for the survival and functionality of many cell types as it helps adapt to changes in metabolic and environmental conditions.

Pathways

NHE-1 integrates into key cellular signaling systems such as the mitogen-activated protein kinase (MAPK) pathway and the Apoptosis signaling pathway. In the MAPK pathway NHE-1 interacts with signaling molecules to influence cellular responses to growth signals. Additionally its regulation of intracellular pH and ionic concentrations intersects with pathways involving proteins like Calcineurin which can impact various cellular responses and adaptations.

NHE-1 is heavily linked to several cardiovascular conditions including hypertension and cardiac hypertrophy. Its dysregulation can alter cardiac cell function contributing to the pathology of these diseases. In cancer aberrant NHE-1 activity may lead to increased cell survival and proliferation. The protein also connects to disorders like ischemia where its function influences the role of related proteins like Na+/K+ ATPase further implying NHE-1’s involvement in pathological states.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism (PubMed : 11350981, PubMed : 11532004, PubMed : 14680478, PubMed : 15035633, PubMed : 15677483, PubMed : 17073455, PubMed : 17493937, PubMed : 22020933, PubMed : 27650500, PubMed : 32130622, PubMed : 7110335, PubMed : 7603840). Exchanges intracellular H(+) ions for extracellular Na(+) in 1 : 1 stoichiometry (By similarity). Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival (PubMed : 12947095, PubMed : 15096511, PubMed : 22020933, PubMed : 8901634). In addition, can transport lithium Li(+) and also functions as a Na(+)/Li(+) antiporter (PubMed : 7603840). SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs (PubMed : 15096511).

See full target information SLC9A1

대체 명칭 보기

APNH1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1, APNH, Na(+)/H(+) exchanger 1, Solute carrier family 9 member 1, NHE-1

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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Basic research in cardiology 119:751-772 PubMed39046464

2024

Empagliflozin prevents heart failure through inhibition of the NHE1-NO pathway, independent of SGLT2.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Chen,Qian Wang,Diane Bakker,Xin Hu,Liping Zhang,Ingeborg van der Made,Anna M Tebbens,Csenger Kovácsházi,Zoltán Giricz,Gábor B Brenner,Peter Ferdinandy,Gert Schaart,Anne Gemmink,Matthijs K C Hesselink,Mathilde R Rivaud,Michael P Pieper,Markus W Hollmann,Nina C Weber,Jean-Luc Balligand,Esther E Creemers,Ruben Coronel,Coert J Zuurbier

Translational andrology and urology 11:1083-1095 PubMed36092839

2022

Propranolol suppresses bladder cancer by manipulating intracellular pH via NHE1.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Hu,Jiali Hu,Cuiyu Chen,Yang Wang,Yu Zhang,Jielin Wan,Ouyang Jing,Hanying Yi,Shiyu Wang,Weihua Huang,Jie Liu,Wei Zhang,Howard L McLeod,Ran Xu,Yijing He

Frontiers in physiology 12:642752 PubMed33868007

2021

Long-Term Angiotensin II Infusion Induces Oxidative and Endoplasmic Reticulum Stress and Modulates Na Transporters Through the Nephron.

Applications

Unspecified application

Species

Unspecified reactive species

Bruna Bezerra Lins,Fernando Augusto Malavazzi Casare,Flávia Ferreira Fontenele,Guilherme Lopes Gonçalves,Maria Oliveira-Souza

Brain research 1763:147448 PubMed33771519

2021

Sex hormones regulate NHE1 functional expression and brain endothelial proteome to control paracellular integrity of the blood endothelial barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Kiera T Blawn,Kathryn L Kellohen,Emily A Galloway,Jared Wahl,Anjali Vivek,Vani G Verkhovsky,Natalie K Barker,Karissa E Cottier,Tissiana G Vallecillo,Paul R Langlais,Erika Liktor-Busa,Todd W Vanderah,Tally M Largent-Milnes

PloS one 15:e0227463 PubMed32469979

2020

Functional NHE1 expression is critical to blood brain barrier integrity and sumatriptan blood to brain uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Liktor-Busa,Kiera T Blawn,Kathryn L Kellohen,Beth M Wiese,Vani Verkhovsky,Jared Wahl,Anjali Vivek,Seph M Palomino,Thomas P Davis,Todd W Vanderah,Tally M Largent-Milnes

Cellular and molecular biology (Noisy-le-Grand, France) 63:11-18 PubMed28719339

2017

Modulation of oxidative and glycolytic skeletal muscle fibers Na+/H+ exchanger1 (NHE1) and Na+/HCO3- co-transporter1 (NBC1) genes and proteins expression in type 2 diabetic rat (Streptozotocin + high fat diet) following long term endurance training.

Applications

Unspecified application

Species

Unspecified reactive species

A Monazzami,H Rajabi,R Ghrakhanlou,K Yari,Z Rahimi

Journal of cellular biochemistry 114:658-68 PubMed23059814

2012

Suppression of mammalian bone growth by membrane transport inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamad Y Loqman,Peter G Bush,Colin Farquharson,Andrew C Hall

The Journal of biological chemistry 286:32563-74 PubMed21705333

2011

Severe neurologic impairment in mice with targeted disruption of the electrogenic sodium bicarbonate cotransporter NBCe2 (Slc4a5 gene).

Applications

IHC-FoFr

Species

Mouse

Liyo Kao,Lisa M Kurtz,Xuesi Shao,Marios C Papadopoulos,Li Liu,Dean Bok,Steven Nusinowitz,Bryan Chen,Salvatore L Stella,Mark Andre,Josh Weinreb,Serena S Luong,Natik Piri,Jacky M K Kwong,Debra Newman,Ira Kurtz

Surgery 149:114-25 PubMed20553904

2010

Sodium hydrogen exchanger as a mediator of hydrostatic edema-induced intestinal contractile dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Karen S Uray,Shinil K Shah,Ravi S Radhakrishnan,Fernando Jimenez,Peter A Walker,Randolph H Stewart,Glen A Laine,Charles S Cox

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody

Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (AB67313)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (9)

Product promise