AB242007

Anti-Sodium Iodide Symporter antibody [FP5]

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(5 제품이 사용된 논문 )

Mouse Monoclonal Sodium Iodide Symporter antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC5A5 aa 450 to C-terminus.

대체 명칭 보기

NIS, SLC5A5, Sodium/iodide cotransporter, Na(+)/I(-) cotransporter, Natrium iodide transporter, Sodium-iodide symporter, Solute carrier family 5 member 5, Na(+)/I(-) symporter

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Formalin-fixed, paraffin-embedded mouse trachea tissue stained for Sodium Iodide Symporter with ab242007 at a 1/1000 dilution for 1 hr at RT in immunohistochemical analysis. Fixation : 10% Formalin Solution for 12-24 hours at RT.

Secondary Antibody : HRP/DAB Detection System : Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain : Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μL for 5 minutes at RT.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Formalin-fixed, paraffin-embedded mouse thyroid tissue stained for Sodium Iodide Symporter with ab242007 at a 1/1000 dilution for 1 hr at RT in immunohistochemical analysis. Fixation : 10% Formalin Solution for 12-24 hours at RT.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Supplier Data

Western blot - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

All lanes:

Western blot - Anti-Sodium Iodide Symporter antibody [FP5] (ab242007) at 1/1000 dilution

All lanes:

Human thyroid lysate

Predicted band size: 68 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

FP5

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SLC5A5 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92911

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium Iodide Symporter also called NIS or SLC5A5 actively transports iodide into cells particularly in the thyroid gland. This protein weighs approximately 87 kDa and primarily locates in the thyroid follicular cells. It exists also in lactating mammary glands salivary glands and gastric mucosa. By concentrating iodide in these tissues NIS plays an important role in synthesizing thyroid hormones which are essential for metabolism regulation.

Biological function summary

NIS facilitates iodide uptake into thyroid cells enabling the synthesis of thyroid hormones triiodothyronine (T3) and thyroxine (T4). This protein doesn't work alone; instead it is a part of a larger thyroid hormone synthesis complex interacting with thyroid peroxidase (TPO) and thyroglobulin (TG) to ensure efficient hormone production. Its action depends on the sodium gradient established by the Na+/K+ ATPase pump which drives active transport against the concentration gradient.

Pathways

NIS forms a critical component in the hypothalamic-pituitary-thyroid (HPT) axis essential for maintaining the body's metabolism and energy balance. This axis forms a feedback loop where thyrotropin-releasing hormone (TRH) and thyroid-stimulating hormone (TSH) regulate the expression and activity of NIS. In the iodine metabolism pathway NIS cooperates with pendrin a chloride/iodide transporter establishing a seamless flow of iodide into thyroid follicles for hormone synthesis.

NIS dysfunction relates to thyroid-related conditions such as congenital hypothyroidism and papillary thyroid cancer. In congenital hypothyroidism NIS mutations reduce iodide uptake leading to insufficient thyroid hormone production. Papillary thyroid cancer might exploit NIS for its malignancy but altered regulation hampers effective radioiodine therapy. In these disorders the interplay with proteins like TPO and TG alters the normal hormone synthesis process causing significant health issues.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Sodium : iodide symporter that mediates the transport of iodide into the thyroid gland (PubMed : 12488351, PubMed : 18372236, PubMed : 18708479, PubMed : 20797386, PubMed : 31310151, PubMed : 32084174, PubMed : 8806637, PubMed : 9329364). Can also mediate the transport of chlorate, thiocynate, nitrate and selenocynate (PubMed : 12488351).

See full target information SLC5A5

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Aging cell 23:e14209 PubMed38825816

2024

Sevoflurane-induced overexpression of extrasynaptic α5-GABAR via the RhoA/ROCK2 pathway impairs cognitive function in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhun Wang,Jinpeng Dong,Mengxue Zhang,Sixuan Wang,Jiangnan Wu,Shengran Wang,Yuan Luo,Yongan Wang,Yiqing Yin

Clinical and translational medicine 14:e1694 PubMed38797942

2024

Targeting miR-31 represses tumourigenesis and dedifferentiation of BRAF-associated thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Peitao Zhang,Lizhao Guan,Wei Sun,Yu Zhang,Yaying Du,Shukai Yuan,Xiaolong Cao,Zhengquan Yu,Qiang Jia,Xiangqian Zheng,Zhaowei Meng,Xingrui Li,Li Zhao

European thyroid journal 12: PubMed37352166

2023

Iodine avidity in papillary and poorly differentiated thyroid cancer is predicted by immunohistochemical and molecular work-up.

Applications

Unspecified application

Species

Unspecified reactive species

Joachim N Nilsson,Jonathan Siikanen,Vincenzo Condello,Kenbugul Jatta,Ravi Saini,Christel Hedman,Catharina Ihre Lundgren,C Christofer Juhlin

Environmental science & technology 57:6072-6084 PubMed37022920

2023

Thyroid-Disrupting Effects of Exposure to Fipronil and Its Metabolites from Drinking Water Based on Human Thyroid Follicular Epithelial Nthy-ori 3-1 Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhui Zhuang,Zhiqiang Jiang,Dawei Chen,Jingguang Li,M James C Crabbe,Meiyue Qiu,Yuxin Zheng,Weidong Qu

Frontiers in pharmacology 12:795386 PubMed35002727

2021

AhR Antagonist Promotes Differentiation of Papillary Thyroid Cancer Regulating circSH2B3/miR-4640-5P/IGF2BP2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ri Sa,Meiliang Guo,Danyan Liu,Feng Guan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sodium Iodide Symporter antibody [FP5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

Western blot - Anti-Sodium Iodide Symporter antibody [FP5] (AB242007)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise