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AB244999

Anti-Sorbitol Dehydrogenase antibody [EPR20780-169] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Sorbitol Dehydrogenase antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-Sorbitol Dehydrogenase antibody [EPR20780-169] - BSA and Azide free (Detector) (AB244999)
  • sELISA

Unknown

Sandwich ELISA - Anti-Sorbitol Dehydrogenase antibody [EPR20780-169] - BSA and Azide free (Detector) (AB244999)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab233613).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20780-169

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB233613

Human Sorbitol Dehydrogenase ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)

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Sample Prep & Detection Kits

AB201804

DyLight® 680 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - DyLight® 680 Conjugation Kit (Fast) - Lightning-Link® (AB201804)

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Sample Prep & Detection Kits

AB270705

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (AB270705)

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Primary Antibodies

AB290676

Anti-APLP1 antibody [EPR25737-6] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-APLP1 antibody [EPR25737-6] - BSA and Azide free (Capture) (AB290676)

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Primary Antibodies

AB259692

Anti-Aspartate Aminotransferase antibody [EPR20781-282] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Aspartate Aminotransferase antibody [EPR20781-282] - BSA and Azide free (Detector) (AB259692)

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Primary Antibodies

AB242996

Anti-Agrin antibody [EPR20025-97] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Agrin antibody [EPR20025-97] - BSA and Azide free (Detector) (AB242996)

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Primary Antibodies

AB315916

Anti-Quiescin Q6 antibody [EPR25653-66] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Quiescin Q6 antibody [EPR25653-66] - BSA and Azide free (Detector) (AB315916)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab244999 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Sorbitol Dehydrogenase. The recommended pair for sandwich ELISA is:
Capture: ab244731, Human Sorbitol Dehydrogenase Capture Antibody (unconjugated)
Detector: ab244999, Human Sorbitol Dehydrogenase Detector Antibody (unconjugated)
The reference range value is 0.39 - 25 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Sorbitol Dehydrogenase (SDH) also known as SORD is an enzyme that catalyzes the oxidation of sorbitol to fructose. This reaction involves the conversion of a secondary alcohol group to a ketone. SDH has a molecular mass of approximately 38 kDa. It is expressed in various human tissues most importantly in the liver kidneys and seminal vesicles. The enzyme requires NAD+ as a cofactor for its catalytic activity.
Biological function summary

SDH plays a part in the polyol pathway which is important for the metabolism of glucose into fructose. It functions alongside aldose reductase to regulate the conversion of glucose into sorbitol and then sorbitol into fructose. SDH acts independently without forming a complex with other proteins. The amount and activity of SDH can affect cellular osmotic balance and energy storage.

Pathways

SDH is involved in the polyol pathway and influences sorbitol levels. An excess of sorbitol can contribute to cellular damage especially in the context of diabetes. SDH’s role is linked to the exclusive conversion of sorbitol to fructose contrasting with aldose reductase which initiates sorbitol production from glucose. Both enzymes are key in maintaining the balance between extracellular and intracellular glucose.

Alterations in SDH activity relate to diabetic complications like diabetic retinopathy and neuropathy. The accumulation of sorbitol due to insufficient SDH activity contributes to the development of these conditions. SDH's connection with aldose reductase is particularly notable here as both proteins impact the sorbitol levels that affect various tissues under hyperglycemic conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively (PubMed : 3365415). Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia (PubMed : 12962626, PubMed : 25105142, PubMed : 29966615). May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (PubMed : 16278369). May have a more general function in the metabolism of secondary alcohols since it also catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol. To a lesser extent, can also oxidize L-arabinitol, galactitol and D-mannitol and glycerol in vitro. Oxidizes neither ethanol nor other primary alcohols. Cannot use NADP(+) as the electron acceptor (PubMed : 3365415).
See full target information SORD

대체 명칭 보기

Sorbitol dehydrogenase, SDH, L-iditol 2-dehydrogenase, Polyol dehydrogenase, Ribitol dehydrogenase, Xylitol dehydrogenase, RDH, XDH, SORD
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Product promise

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