Anti-SOS1 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-SOS1 antibody (AB245645)

SOS1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

ab245645 used for IP at 3 μg/mg lysate. For WB 1 μg/ml.

Lane 1 : ab245645 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-SOS1 antibody (ab245645)

Predicted band size: 152 kDa

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• WB

Lab

Western blot - Anti-SOS1 antibody (AB245645)

False colour image of Western blot : Anti-SOS1 antibody staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab245645 was shown to bind specifically to SOS1. A band was observed at 171 kDa in wild-type A431 cell lysates with no signal observed at this size in SOS1 knockout cell line ab276087 (knockout cell lysate ab283833). To generate this image, wild-type and SOS1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-SOS1 antibody (ab245645) at 1/2000 dilution

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

SOS1 knockout A431 cell lysate at 20 µg

Lane 2:

Western blot - Human SOS1 knockout A-431 cell line (<a href='/ko/products/cell-lines/human-sos1-knockout-a-431-cell-line-ab276087'>ab276087</a>)

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

SK-OV-3 cell lysate at 20 µg

Predicted band size: 152 kDa

Observed band size: 171 kDa

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• WB

Supplier Data

Western blot - Anti-SOS1 antibody (AB245645)

All lanes:

Western blot - Anti-SOS1 antibody (ab245645) at 0.04 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 152 kDa

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Exposure time: 3min