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AB325591

Anti-SOS1 (phospho Y1196) antibody [TF7-28-23]

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Mouse Recombinant Monoclonal SOS1 phospho Y1196 antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Human, Synthetic peptide - Human samples.
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Indirect ELISA - Anti-SOS1 (phospho Y1196) antibody [TF7-28-23] (AB325591)
  • I-ELISA

Lab

Indirect ELISA - Anti-SOS1 (phospho Y1196) antibody [TF7-28-23] (AB325591)

Indirect ELISA analysis of ab325591 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution dilution.

Antigen : Human SOS1 (phospho Y1196) peptide;Human SOS1 non-phospho peptide.

Antigen concentration : 1000 ng/ml

Western blot - Anti-SOS1 (phospho Y1196) antibody [TF7-28-23] (AB325591)
  • WB

Lab

Western blot - Anti-SOS1 (phospho Y1196) antibody [TF7-28-23] (AB325591)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Loading Control (ab176842) (1 : 100000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(150-250kDa).

All lanes:

Western blot - Anti-SOS1 (phospho Y1196) antibody [TF7-28-23] (ab325591) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with a human wild-type SOS1 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with a human SOS1 (Y1196A mutation) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 152 kDa,36 kDa,150-250 kDa

true

Exposure time: 180s

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

TF7-28-23

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects transfected levels of human SOS1 protein only.

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보완 제품

Reagents, Controls & Accessories

AB156035

Native lysis Buffer

SDS-PAGE - Native lysis Buffer (AB156035)

  • 5
  • 1
Primary Antibodies

AB11826

Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

  • 4
  • 16
Assay Kits

AB113851

DCFDA / H2DCFDA - Cellular ROS Assay Kit

Functional Studies (Act) - DCFDA / H2DCFDA - Cellular ROS Assay Kit (AB113851)

  • 5
  • 11
Primary Antibodies

AB215191

Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "125 ng/mL", "IELISA-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Promotes the exchange of Ras-bound GDP by GTP (PubMed : 8493579). Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3/ERK1 in response to EGF (PubMed : 17339331). Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity (By similarity).
See full target information SOS1 phospho Y1196

대체 명칭 보기

Son of sevenless homolog 1, SOS-1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com